2,2-dimethyl-1,3-propanediyl didecanoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 - 15 Dec 2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (purity not reported, no analytical monitoring)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fraction (WAF): This WAF was prepared by moderately stirring 100 mg test substance per liter of medium overnight
followed by filtration (MILLIPORE AP15 glass fiber filter).
- Controls: yes, water controls

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Length at study initiation: < 2mm
- Method of breeding: Parental and young Daphnia were held in 40L glass aquaria with a frequency of feeding once a day, except weekends, of 15 g sera micropan (homogenised in 1 L deionised water). The temperature was 20 ± 2 °C with an illumination of 16 h per day.
- Feeding during test: none

ACCLIMATION
- Acclimation period: 4 hours
- Acclimation conditions (same as test or not): same as test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

20.0 ± 0.5 °C

pH:

control: 7.5 - 7.6
100 mg/L: 6.7 - 7.5

Dissolved oxygen:

control: 6.0 mg/L
100 mg/L: 5.8 - 6.0 mg/L

Nominal and measured concentrations:

Nominal: 0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all-glass, 100 mL flasks, with 50 mL of test medium
- Aeration: no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water according to ISO 6341, prepared with distilled water.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
- Culture medium different from test medium: No, but test medium was not aerated as the culture medium.
- Intervals of water quality measurement: Temperature determined in a control flask at the start and at the end of the test. Oxygen and pH were
determined in all vessels prior to the addition of Daphnia to the test medium and at the end of the test.

OTHER TEST CONDITIONS
- Photoperiod: 16 h a day, supplied by overhead white fluorescent tubes.

EFFECT PARAMETERS MEASURED: Observations of immobile Daphnia were made after 24 and 48 h of exposure.

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EL0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Table 1: Acute immobilisation of Daphnia magna after 24 and 48 hours of exposure

Nominal concentration (mg/l)	Code	Number of individuals per test vessel	Immobile Daphnia (No./vessel) 24h	Immobile Daphnia (No./vessel) 48h	% immobile Daphnia after 24h of exposure	% immobile Daphnia after 48h of exposure
Control 0	A B C	20 20 20	0 0 0	0 0 0	0 0 0	0 0 0
100	A B	20 20	0 0	0 0	0 0	0 0

 

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

Summary of available data used for the endpoint assessment of the target substance

Adequacy of study:

key study

Justification for type of information:

Refer to analogue justification document provided in IUCLID section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

source, CAS 85186-86-3, Oleon, 2007, D. magna 48 h, RL2

Duration:

48 h

Dose descriptor:

EL0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

source, CAS 85186-86-3, Oleon, 2007, D. magna 48 h, RL2

Conclusions:

No effects were observed up to the limit of water solubility.

Description of key information

EL50 (48h) > 100 mg/L (nominal; Daphnia magna); no effects up to the limit of water solubility; read-across

Additional information

There is no study available in which the short-term toxicity of Neopentyl glycol dicaprate (CAS No. 27841-06-1) to aquatic invertebrates was assessed. Therefore, read-across to the structurally and chemically closely related source substance Fatty acids, C8-18 and C18-unsatd., esters with neopentyl glycol (CAS 85186-86-3) was performed in accordance with Regulation (EC) No 1907/2006 Annex XI, section 1.5 in order to fulfill the data requirements for this substance. The source substance is characterized by similar chemical structure and is therefore considered a suitable representative for the assessment of the short-term toxicity of the target substance to aquatic invertebrates. A detailed read-across justification in provided in IUCLID section 13.

The study conducted with the source substance Fatty acids, C8-18 and C18-unsatd., esters with neopentyl glycol (CAS 85186-86-3) was conducted under static conditions and according to OECD Guideline 202, usingDaphnia magnaas test organism. A 100 mg/L loading rate, prepared as Water Accommodated Fraction (WAF), was applied in a limit test. No immobility was observed in the control and the treatment during the 48 h exposure period. Hence, the EL50 (48 h) was determined as > 100 mg/L loading rate (WAF, nominal). No effects up to the limit of water solubility are expected. Based on the available results from a structurally related read-across substance (in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5) which is characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that the target substance Neopentyl glycol dicaprate (CAS No. 27841-06-1) is not actuely toxic to aquatic invertebrates.