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EC number: 219-014-5 | CAS number: 2314-97-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb 10, 2000 to Mar 31, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Supplier: F-Tech, Inc.
- Purity:>99%
- Impurity: Trifluoromethane <1%
- Lot No. 990423
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: 1484mg/L, stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: does not react. - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Returned sludge from sewage plant was collected.
- Storage conditions: Stored unde aerobic condition
- Water filtered: yes
- Type and size of filter used, if any: FIlter No. 2 - Duration of test (contact time):
- 28 d
- Initial conc.:
- 3.64 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Prepared the following stock solutions, using analytical grade reagents:
(a) Potassium dihydrogen orthophosphate, KH2PO4 8.5 g
Dipotassium hydrogen orthophosphate K2HPO4 21.75 g
Disodium hydrogen orthophosphate dihydrate, Na2HPO4.2H2O 33.40g
Ammonium chloride, NH4Cl 0.5g
Dissolve in water and make up to 1 litre. The pH of the solution should be 7.4.
(b) Calcium chloride, anhydraous, CaCl2 27.5g
or
Calcium chloride dihydrate, CaCl2.2H2O 36.40g
Dissolve in water and make up to 1 litre.
(c) Magnesium sulphate hyptahydrate, MgSO4.7H2O 22.50 g
Dissolve in water and make up to 1 litre.
(d) Iron(III) chloride hexahydrate, FeCl3.6H2O 0.25 g
Dissolve in water and make up to 1 litre.
Mix 1 ml of solutions (a),(b), (c) and (d) with water and make up to 1 litre.
- Solubilising agent (type and concentration if used):
The saturated solution of test substance was prepared by bubbling test substance into 50 ml water for 60 min.
The concentration of the test substance in saturated solution was 1484 mg/L.
Concentration of the test substance 3.6mg/L
- Test temperature: 20±1 °C
- pH adjusted: no
- Aeration of dilution water: No
- Continuous darkness: yes
- Other: Closed bottle
TEST SYSTEM
- Culturing apparatus: 102 ml culturing bottle
- Number of culture flasks/concentration: 12 (test sample), 4 (reference), 11 (blank)
- Test performed in closed vessels due to significant volatility of test substance: Yea
- Test performed in open system: For reference substance, aniline
- Analysis : Dissolved oxygen measurement: Titration: Winkler azide modification method.
- Other:
SAMPLING
- Sampling frequency: 7, 14,21 and 28 day
CONTROL AND BLANK SYSTEM
- Inoculum blank: 11 - Reference substance:
- aniline
- Test performance:
- Validity of this test was confirmed as follows;
1) The percentage degradation of the reference compound was 78% calculated by BOD after 14 days.
2) Oxygen depletion in the inoculum blank did not exceed 1.5 mg dissolved oxygen/L after 28 days.
3) The residual concentration of oxygen in the test tube did not fall below 0.5 mg/L. - Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- >= 0 - <= 2
- Sampling time:
- 28 d
- Key result
- Parameter:
- other: % degradation (BOD)
- Value:
- >= 0 - <= 2
- Sampling time:
- 28 d
- Parameter:
- other: % degradation (BOD)
- Value:
- 0
- Sampling time:
- 21 d
- Parameter:
- other: % degradation (BOD)
- Value:
- >= 0
- Sampling time:
- 14 d
- Parameter:
- other: % degradation (BOD)
- Value:
- >= 1 - <= 2
- Sampling time:
- 7 d
- Details on results:
- Recovery test and blank test for test substance
1) Recovery test
Water + test substance 97.0 % 99.2 % average 98.1 %
Sludge + test substance 96.1 % 99.4 % average 97.7 %
2) Blank test
Test substance peak was not observed with GC analysis in the blank test. - Results with reference substance:
- Referenc substance: Aniline
Percentage biodegradation after 7 days 82 % 83% average 82%
Percentage biodegradation after 14 days 77 % 79% average 78% - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under test condition, the test substance was not ready biodegradable.
- Executive summary:
The screening of chemicals for ready biodegradability of the test substance was carried out with closed bottle method (OECD 301D). Under the test condition, the test substance was not ready biodegradable.
Reference
Table 1-1 Calculation table for dissolved oxygen measurements (Closed Bottle Test)
Sample description |
Time of incubation (After x day) |
||||
0 |
7 |
14 |
21 |
28 |
|
Control blank |
9.24 |
9.08 |
8.78 |
8.58 |
8.42 |
Control blank |
9.26 |
9.12 |
8.84 |
8.68 |
8.54 |
Average (mb) |
9.25 |
9.10 |
8.81 |
8.63 |
8.48 |
|
|
|
|
|
|
Sludge + test substance (mt1) |
- |
8.98 |
8.82 |
8.66 |
8.38 |
Sludge + test substance (mt2) |
- |
9.02 |
8.92 |
8.74 |
8.46 |
|
|
|
|
|
|
Sludge + sodium benzoate(mt1) |
- |
5.00 |
4.94 |
- |
- |
Sludge + sodium benzoate(mt2) |
|
4.96 |
4.86 |
- |
- |
Table 1-2 Calculation table for the percentage biodegradation by BOD (Closed Bottle Test)
Sample description |
After X day |
C |
D |
E |
Sludge + test substance -1 |
7 |
0.12 |
2 |
|
-2 |
7 |
0.08 |
1 |
2 |
-3 |
14 |
0.00 |
0 |
|
-4 |
14 |
0.00 |
0 |
0 |
-5 |
21 |
0.00 |
0 |
|
-6 |
21 |
0.00 |
0 |
0 |
-7 |
28 |
0.10 |
2 |
|
-8 |
28 |
0.02 |
0 |
1 |
Sludge + sodium benzoate -1 |
7 |
4.10 |
82 |
|
-2 |
7 |
4.14 |
83 |
82 |
-3 |
14 |
3.87 |
77 |
|
-4 |
14 |
3.95 |
79 |
78 |
A: Concentration (mg/L)
Test substance 3.64 (mg/L)
Sodium benzoate 3.00 (mg/L)
B: TOD (mgO2/L)
TOD of test substance
CF3I + 10O2= CO2+ 3F + I
10O2/CF3I = 319.99/195.91 = 1.63
TOD = 3.64×1.63 = 5.95 (mgO2/L)
TOD of sodium benzoate
C7H5NaO2+ 7.5O2= 7CO2+ 2.5H2O + 0.5Na2O
7.5O2/C7H5NaO2= 239.99/144.1 = 1.67
TOD = 3.00×1.67 = 5.01 (mgO2/L)
C: BOD after x days (mgO2/L) = mb(x) – mt(x) (see table 1-1)
D: Percentage of biodegradation (%) = C/B×100
E: Average percentage of biodegradation (%)
Table 2 Calculation table for recovery rate by GC
Sample description |
A |
D |
E |
F |
Standard solution 0.0576 mg/L |
3367 |
|
|
|
Water + test substance – 1 |
2994 |
0.366 |
97.0 |
|
Water + test substance – 2 |
3063 |
0.374 |
99.2 |
98.1 |
Sludge + test substance – 1 |
2965 |
0.362 |
96.1 |
|
Sludge + test substance – 2 |
3067 |
0.375 |
99.4 |
97.7 |
Control blank |
n.d. |
|
|
|
Amount of test substance added: 0.377 (mg)
A: Peak area (μV·sec)
B: Final volume: 70 (mL)
C: Ratio of portion used for analysis: 1/102
D: Recovery amount (mg)
D = G×(A/A(Standard))×(B/C)/1000
E: Recovery rate (%)
E = D/0.377 (mg)×100
F: Average recovery rate (%)
G: Concentration of standard solution: 0.0576 (mg/L)
Table 3 Calculation table for percentage biodegradation by GC
Sample description |
A |
E |
F |
G |
H |
Standard solution 0.0576mg/L |
3248 |
|
|
|
|
Water + test substance -1 |
2832 |
0.359 |
97 |
|
|
Water + test substance -2 |
2922 |
0.370 |
100 |
|
|
Sludge + test substance -1 |
2884 |
0.365 |
98 |
0 |
|
Sludge + test substance -2 |
2817 |
0.365 |
96 |
2 |
1 |
Control blank |
n.d. |
|
|
|
|
Amount of test substance added: 0.371 (mg)
A: Peak Area (μV·sec)
B: Final volume: 70(mL)
C: Ratio of portion used for analysis: 1/102
D: Recovery rate: 98.1 (%) (Water + test substance)
97.7 (%) (Sludge + test substance)
E: Residual amount of test substance (mg)
E = I×(A/A(standard))×(B/C)/(D/100)/1000
F: Percentage residue (%)
F = E/0.371 (mg)×100
G: Percentage biodegradation (%)
G = {(E(Water + test substance) – E(Sludge + test substance))/E(Water + test substance)}×100
H: Average percentage biodegradation (%)
I: Concentration of standard solution: 0.0576 (mg/L)
Description of key information
The substance is not readily biodegradable as observed in a biodegradation screening study according to OECD TG 301D
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The biodegradation potential of the substance in water was determined in a screening study according to OECD TG 301D (Closed Bottle Test) and in compliance with GLP criteria (Kurume Laboratory, 2001). In this study 3.64 mg/L test substance was inoculated with returned sludge (activated, non-adapted) from sewage plant for 28 days under aerobic conditions in the dark. After the 28-day incubation period, the cumulative percentages of biodegradation were calculated from both the oxygen depletion (biological oxygen demand; BOD) and from the decrease of test item concentrations. The observed biodegradation percentages did not exceed 2% in either of the two analysis methods. Based on these findings the substance is classified as not readily biodegradable.
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