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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August 2006 - June 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
- Name: Metaldehyde
- Physical appearance: crystalline white powder
- Storage requirements: dry and cool at a well ventilated place according to MSDS provided by the sponsor
- Stability under correct storage conditions/expiry date: 15 July 2009
Analytical monitoring:
yes
Details on sampling:
Sampling procedure:
- at the beginning of the test, duplicate samples of the stock solution will be taken
- at the beginning of the study duplicate samples of the control, and treated test solutions will be taken from the volumetric flasks
- at the end of the test immediately after determination of the biological and physicochemical parameters, the test solutions of the replicates of a given concentration will be mixed. Duplicate samples will be taken from controls and treated vessels, and from test vessels without algae at the highest test ttem concentration
Vehicle:
no
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Remarks on exposure duration:
standard test duration
Post exposure observation period:
none
Hardness:
not mentioned
Test temperature:
22.8 +/- 0.24°C
pH:
7.5
Dissolved oxygen:
no data
Salinity:
freshwater used
Conductivity:
no data
Nominal and measured concentrations:
The mean total recovery for all measured test solutions was 99.3% of the nominal concentration. Therefore a correction of the biological results based on measured concentrations was not perfonned.
- Test concentrations: 200, 100, 50, 25, and 12.5 mg test item/L plus a control
- Number of replicates per test concentration: 3 for test item concentrations, and 6 for control
Details on test conditions:
- Test organisms: Desmodesmus subspicatus (SAG 86.81)
- Supplier: Sammlung für Algenkulturen (SAG), Albrecht-von-Haller lnstitut für Pflanzenwissenschaften der Universität Göttingen, D-37073 Göttingen, Germany
- Test medium: US-EPA medium AAP
- Light regime: Permanent
- Light intensity: 76.4 uE/m2 • s (required: 60-120 u/m2 • s)
- Test units: 50 mL Erlenmeyer flasks



Reference substance (positive control):
not specified
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: EC50 for yield and growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield
Details on results:
After an exposure period of 72 hours, the EC50 for growth rate and yield was determined to be higher than 200 mg test item/L.
Results with reference substance (positive control):
not tested
Reported statistics and error estimates:
- The statistical software package ToxRat 2.09 Professional (ToxRat Solutions GmbH, Naheweg 15, D-524 77 Alsdorf) was used for these calculations.
- Probit analysis (FINNEY, 1971) was used to calculate the ECx values. Where the calculated ECx values were extrapolated to concentrations above the highest tested concentration, the respective ECx values are reported as "> 200 mg/L".
- The Range-To-Standard-Deviation-Ratio-Test was used to test correspondence of the data with normal distribution. The data were evaluated for homogeneity of variances by Cochran's Test. One-way Analysis of Variance (ANOVA) and subsequent multi-comparison tests (Williams' Test) were used to calculate whether there were significant differences between the growth of algae in the controls and the algae exposed to the various test item concentrations.
Validity criteria fulfilled:
yes
Conclusions:
After an exposure period of 72 hours, the EC50 for growth rate and yield was determined to be higher than 200 mg test item/L.
Executive summary:

The acute toxicity of the test item to green algae (Desmodesmus subspicatus) was determined in a 72 -hour static test according to the OECD test method no. 201. A full test with five test item concentrations (200, 100, 50, 25 and 12.5 mg/L) was performed in accordance with the test guidelines. The mean total recovery for all measured test solutions was 99.3% of the nominal concentration. Therefore a correction of the biological results based on measured concentrations was not performed. After an exposure period of 72 hours, the EC50 for growth rate and yield was determined to be higher than 200 mg test item/L.

Description of key information

The acute toxicity of the test item to green algae (Desmodesmus subspicatus) was determined in a 72 -hour static test according to the OECD test method no. 201. A full test with five test item concentrations (200, 100, 50, 25 and 12.5 mg/L) was performed in accordance with the test guidelines. The mean total recovery for all measured test solutions was 99.3% of the nominal concentration. Therefore a correction of the biological results based on measured concentrations was not performed. After an exposure period of 72 hours, the EC50 for growth rate and yield was determined to be higher than 200 mg test item/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
200 mg/L
EC10 or NOEC for freshwater algae:
25 mg/L

Additional information

Source: GLP-report