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EC number: 202-700-3 | CAS number: 98-79-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 November 2016 to 29 Januray 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot no. of test material: 20140124
- Expiration date of the lot/batch: 22 May 2019
- Purity test date: 22 May 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient temperature
- Stability under test conditions: No data - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control, 6.25, 12.5, 25, 50 and 100 mg/L
- Sampling method: Four replicate samples (triplicate samples for analysis and 1 reserve sample) of freshly prepared test solutions at 0h and
- Sample storage conditions before analysis: - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg/L nominal test solution prepared in Elendt M4 medium. This test solution was used to prepare remaining test solutions by dilution
- Eluate: Elendt M4
- Controls: Test medium only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No data - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: CEMAS laboratory culture
- Justification for species other than prescribed by test guideline: Not applicable
- Source: CEMAS laboratory
- Age of parental stock (mean and range, SD): No data
- Feeding during test: No
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Daphnia neonates were <24h old at the start of the test and were not 1st brood progeny - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- No data
- Test temperature:
- 20 +/- 2oC
- pH:
- 0h (new): 5.6 - 7.8
24h (aged): 6.8 - 7.7
24h (new): 5.7 - 7.9
48h (aged): 6.7 - 7.7 - Dissolved oxygen:
- 0h (new): 9.37 - 9.63
24h (aged): 8.35 - 9.07
24h (new): 9.31 - 9.40
48h (aged): 8.24 - 9.04 - Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal test concentrations: Control and 6.25, 12.5, 25, 50 and 100 mg/L
Geometric mean measured test concentrations (48h): Control and 2.97, 9.13, 22.2, 46.9 and 94.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open (covered with Perspex sheet to allow air circulation)
- Material, size, headspace, fill volume: 100 mL glass beakers, filled with 60 mL test solution
- Aeration: None
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon: No data
- Particulate matter: No data
- Metals: No data
- Pesticides: No data
- Chlorine: No data
- Alkalinity: No data
- Ca/mg ratio: No data
- Conductivity: No data
- Salinity: No data
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- Separate study using ptoassium dichromate; 48h EC50 determined as 1.35 mg/L (95% CI 1.31 - 1.45 mg/L); result in line with published data for potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 94.14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 94.14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 94.14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: None observed (compared to control)
- Mortality of control: <10% (0% observed)
- Other adverse effects control: None observed
- Abnormal responses: None observed
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None observed - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 (immobility) was determined to be >94.1 mg/L under the conditions of the test
- Executive summary:
In a 48-hour study the acute toxicity of the aquatic invertebrateDaphnia magnawas studied. The Daphnia were exposed to a range of test concentrations under semi-static conditions according to the OECD test guideline 202 and in compliance with GLP. The nominal test concentrations were a control and 6.25, 12.5, 25, 50 and 100 mg/L, with geometric mean measured test concentrations of 2.97, 9.13, 22.2, 46.9 and 94.1 mg/L, respectively.
No immobility was observed at any test concentration, therefore, the 48-hour EC50 (immobility) was determined to be >94.1 mg/L geometric mean measured concentration.
This acute toxicity study is considered acceptable and satisfied the guideline requirements for an aquatic invertebrate short-term toxicity test.
Reference
Immobilisation forDaphnia magna(initial population 20) |
|||||
Nominal concn (mg/L) |
Geometric mean measured concn (mg/L) |
Observation period |
|||
24-hour |
48-hour |
||||
Absolute no. immobilised |
Cumulative no. immobilised |
Absolute no. immobilised |
Cumulative no. immobilised |
||
Control (0) |
- |
0 |
0 |
0 |
0 |
6.25 |
2.97 |
0 |
0 |
0 |
0 |
12.5 |
9.13 |
0 |
0 |
0 |
0 |
25 |
22.2 |
0 |
0 |
0 |
0 |
50 |
46.9 |
0 |
0 |
0 |
0 |
100 |
94.1 |
0 |
0 |
0 |
0 |
Description of key information
A reliable 48-hour study the acute toxicity of the aquatic invertebrateDaphnia magna was conducted. The Daphnia were exposed to a range of test concentrations under semi-static conditions according to the OECD test guideline 202 and in compliance with GLP.
No immobility was observed at any test concentration, therefore, the 48-hour EC50 (immobility) was determined to be >94.1 mg/L based on geometric mean measured concentrations.
This acute toxicity study is considered acceptable and satisfied the guideline requirements for an aquatic invertebrate short-term toxicity test
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 94.1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.