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Diss Factsheets
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EC number: 947-854-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1992
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 2.2 and 5 mg/L
- Sampling method: 20 mL samples were taken from exposure vessels (at test start, after 48 and 96 h). - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test item was directly weighed into a beaker. Diluation water was added and the mixture was homogenized using an Ultra-Turrax and poured into the chamber under stirring with a glass rod.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: Charles River Aquatics, Broekman Instituut B.V., Somere, The Netherlands (delivery date: 22 Apr 1996; date of hatching: 31 Jan 1996)
- Length at study initiation (length definition, mean, range and SD): 2.8 - 3.0 cm (mean: 2.9 cm; n=21)
ACCLIMATION
- Acclimation period: 12 d
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: Tetra Min, Melle, Germany
- Feeding frequency during acclimation: twice daily ad libitum
FEEDING DURING TEST
- None - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2.3 - 2.5 mmol Ca2+ + Mg2+/L
- Test temperature:
- 21.2 - 22.2 °C
- pH:
- 7.6 - 8.2
- Dissolved oxygen:
- 7.5 - 9.9 mg O2/L
- Nominal and measured concentrations:
- Nominal: control, 1.0, 2.2, 5.5 mg/L
measured: < 1, not analysed, 1.87, 4.67 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria (length: 30 cm, width: 22 cm, height: 24 cm); calibrated to 10 L
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1 (three in total: see "Any other information on materials and methods incl. tables")
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (composition according to ISO/DIS 7346/1)
- Culture medium different from test medium: same as test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light/12 h dark
- Light intensity: approx. 700 lux
EFFECT PARAMETERS MEASURED
- Letahlity and visible changes in appearance and behaviour were assessed after 3, 6, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 2.2
- Range finding study: A range-finding study was conducted but the details were not reported. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Remarks:
- Geometric mean of LC0 and LC100
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No behavioural changes in the control were observed. At 1 mg/L some fish showed increased fright reaction, projecting opercula and were observed swimming posture tail-heavy at the beginning of the study (3+6 h). At 2.2 mg/L some fish showed projecting opercula, increased respiration rate, irregular respiration, hypoactivity and were observed swimming posture tail-heavy throughout exposure. At 5 mg/L some fish showed projecting opercula, dark colored integument, irregular respiration, hypoactivity and were observed swimming posture tail-heavy at the beginning of the study (3+6 h) before they were observed dead.
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Test solutions were clear and colorless with particulate matter at the water surface and on the bottom. - Reported statistics and error estimates:
- Probit analysis could not be performed in order to derive the LC50. Thus, the LC50 was calculated based on the geometric mean of LC0 and LC100.
- Sublethal observations / clinical signs:
Table 1: Lethality of fish during exposure
Concentration [mg/L]
Lethality after x h
3 h
6 h
24 h
48 h
72 h
96 h
0
0/7
0/7
0/7
0/7
0/7
0/7
1
0/7
0/7
0/7
0/7
0/7
0/7
0
0/7
0/7
0/7
0/7
0/7
0/7
2.2
0/7
0/7
0/7
0/7
0/7
0/7
0
0/7
0/7
0/7
0/7
0/7
0/7
5
0/7
0/7
7/7
7/7
7/7
7/7
Reference
Description of key information
LC50 (96 h): 3.3 mg/L (arithmetic mean measured, OECD 203)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.3 mg/L
Additional information
One experimental study is available investigating the short-term toxicity of Fatty acids C16-18 (even numbered), mono and diesters with sucrose (no CAS) to fish. The study was performed according to OECD 203 (GLP) with the zebrafish Danio rerio. Nominal concentrations of 1.0, 2.2 and 5.5 mg/L were tested in a static exposure regime. Analysis by HPLC/UV revealed that the substance concentrations remained stable during exposure (within 80-120% of nominal). Thus, the effect concentrations were based on nominal concentrations. 100% mortality was recorded at 5.5 mg/L whereas 0% mortality was recorded at 2.2 mg/L. Thus, the LC50 (96 h) of 3.3 mg/L (geometric mean of nominal substance concentration) was derived as the geometric mean of the LC0 (1.88 mg/L) and LC100 (4.67 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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