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Diss Factsheets
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EC number: 947-854-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
- Justification for type of information:
- 1. SOFTWARE
Vega version 1.1.3
2. MODEL (incl. version number)
Ready Biodegradability Model (IRFMN) v. 1.0.9
3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
See “Test material information”
4. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
See attached information on the model provided by the developer. Further information on the OECD criteria as outlined by the applicant is provided below under "Any other information of materials and methods incl. tables"
5. APPLICABILITY DOMAIN
See attached information and information as provided in "Any other information on results incl. tables".
6. ADEQUACY OF THE RESULT
See assessment of adequacy as outlined in the "Overall remarks, attachments" section. - Principles of method if other than guideline:
- - Software tool(s) used including version: Vega v1.1.3
- Model(s) used: Ready biodegradability model (version 1.0.9)
Full reference and details of the used formulas can be found in:
Meylan W.M., Howard P.H., Boethling R.S. et al. Improved Method for Estimating Bioconcentration / Bioaccumulation Factor from Octanol/Water Partition Coefficient. 1999, Environ. Toxicol. Chem. 18(4): 664-672
- Model description: see field 'Justification for non-standard information', 'Attached justification' and 'any other information on Material and methods'
- Justification of QSAR prediction: see field 'Justification for type of information', 'Attached justification' and/or 'overall remarks' - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Remarks on result:
- readily biodegradable based on QSAR/QSPR prediction
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 9439: Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sewage treatment plant RWZI Weurt, The Netherlands
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 30 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 20-25 °C
- Aeration of dilution water:
- Suspended solids concentration: 30 mg activated sludge solids/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 L test vessels
- Number of culture flasks/concentration: 2
- Measuring equipment: The carbon dioxide produced was quantified by titration with hydrochloric acid solution.
SAMPLING
- Sampling frequency: On day 1, 5, 12, 16, 23 and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, two bottles
- Abiotic sterile control: no
- Toxicity control: no
- Other: Reference substance: yes, two bottles - Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 56.4
- Sampling time:
- 28 d
- Remarks on result:
- other: Substance A; Mean of two replicates
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 79.1
- Sampling time:
- 28 d
- Remarks on result:
- other: Substance B; Mean of two replicates
- Results with reference substance:
- The reference substance was degraded to 50.1% after 12 d and 60.95% after 16 d. No information is available if the degradation passed the 60% level of degradation after 14 d.
- Validity criteria fulfilled:
- not specified
- Remarks:
- The validity criteria stipulated in the OECD Guideline cannot be checked completely since information are missing.
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Return sludge from Kogasaki municipal sewage treatment plant (date of receipt 06 Aug 1992)
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 24 - 26 °C
- pH: 6.4 - 7.0
TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus
- Number of culture flasks/concentration: 3
- Measuring equipment: Closed system oxygen consumption measuring apparatus (Ohkura Electric Co.); DOC was measured in the supernatant after centrifulation (1000 xg, 10 min); DOC was measured using a TOC analyzer
- Test performed in closed vessels: yes
SAMPLING
- Sampling frequency: BOD was measured continuously; DOC was measured at test end
- Sampling method: For the DOC measurement 10 mL of test solution were removed at test end (centrifuged at 1000 xg for 10 min).
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, one bottle
- Abiotic sterile control: no
- Toxicity control: no
- Other: reference substance: yes, one bottle - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 85.7
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean of three replicates
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean of three replicates
- Results with reference substance:
- The reference substance was degraded to 58% after 14 d and 60% after 28 d based on O2 consumption.
- Validity criteria fulfilled:
- not specified
- Remarks:
- Fulfilment of validity criteria not indicated in the report.
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
For detailed information on the results please refer to the attached report.
Table 1: Degradation of the two test items and the reference substance
Substance |
Biodegradation [%] |
Mean [%] |
|||||
Day 1 |
Day 5 |
Day 12 |
Day 16 |
Day 23 |
Day 28 |
||
|
|
|
|
|
|
|
|
A_Repl. 1 |
0.2 |
20.7 |
35.2 |
58.5 |
66.4 |
70.0 |
58.85 |
A_Repl. 2 |
0.0 |
11.8 |
18.1 |
32.9 |
38.1 |
47.7 |
|
|
|||||||
B_Repl. 1 |
2.4 |
31.8 |
52.1 |
68.7 |
74.8 |
79.2 |
79.05 |
B_Repl. 2 |
3.8 |
30.5 |
49.8 |
68.9 |
74.1 |
78.9 |
|
|
|||||||
Reference_Repl. 1 |
2.3 |
34.6 |
48.8 |
61.5 |
64.2 |
68.0 |
68.05 |
Reference_Repl. 2 |
1.8 |
39.1 |
51.4 |
60.7 |
64.3 |
68.1 |
Table 1: Biodegradation of the test item and reference substance base on BOD
Bottle No. |
ThOD [mg] |
Degradability based on BOD |
|||||||
Day 7 |
Day 14 |
Day 21 |
Day 28 |
||||||
BOD [mg] |
% degradation |
BOD [mg] |
% degradation |
BOD [mg] |
% degradation |
BOD [mg] |
% degradation |
||
1 |
90.2 |
44.2 |
48 |
54.5 |
58 |
56.7 |
60 |
58.2 |
60 |
2 |
- |
1.3 |
- |
2.5 |
- |
3.0 |
- |
3.7 |
- |
3 |
60.2 |
28.3 |
45 |
41.4 |
65 |
50.6 |
79 |
56.1 |
87 |
4 |
60.2 |
28.1 |
45 |
41.4 |
65 |
51.9 |
81 |
56.2 |
87 |
5 |
60.2 |
27.9 |
44 |
40.9 |
64 |
49.3 |
77 |
53.4 |
83 |
6 |
60.2 |
0.0 |
- |
0.0 |
- |
0.0 |
- |
0.5 |
- |
1: Reference substance
2: Activated sludge + basal medium
3 -5: Test substance
6: Test substance + deionized water
Description of key information
The substance is readily biodegradable according to the OECD criteria.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The biodegradation of the substance was assessed in a
Weight-of-Evidence approach. Two experimental studies and QSAR
calculations were assessed for a final conclusion.
The first study was performed according to OECD 301B using domestic
activated sludge as inoculum. The initial test substance concentration
of 30 mg/L was degraded by 56.4% (replicate A) and by 79.1% (replicate
B) after 28 d. Formally the validity criteria of the guideline are not
met since the difference in degradation slightly exceeds the limit of
20%. However, the mean degradation value of 68.95% serves as indication
that the substance is readily biodegradable according to the OECD
criteria.
A second experimental study is available which was performed
according to OECD 301C. DOC removal and O2 consumption were followed for
28 d in order to assess the ready biodegradability of the substance. The
activated sludge was obtained from a sewage treatment plant treating
predominantly domestic sewage. After 28 d the substance was degraded by
85.7% based on O2 consumption and by 99% based on DOC removal.The
available data do not allow assessing the 10-day window criterion.
However, since the substance is a UVCB substance and consists of
structurally similar constituents with e.g. different chain-lengths,
degree and/or site of branching or stereo-isomers], sequential (instead
of concurrent) biodegradation of the individual structures can take
place, but all constituent can be considered as readily biodegradable.
Thus, referring to Annex I of the OECD Guideline for Testing of
Chemicals ‘Revised introduction to the OECD guidelines for testing of
chemicals, section 3’ (OECD, March 2006), the 10-day window should not
be considered for this UVCB substance and due to a degradation of >60 %
within 28 days the substance can be regarded as readily biodegradable.
The results of the experimental studies is further supported by reliable
QSAR calculations for representative constituents of the UVCB substance
(VEGA 1.1.3, Ready biodegradability model v1.0.9). Based on this result
all constituents are considered to be readily biodegradable.
All information (experimental and QSAR) give clear evidence that the
substance is readily biodegradable according to the OECD criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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