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EC number: 216-835-0 | CAS number: 1678-91-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-11-30 - 2018-07-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethylcyclohexane
- EC Number:
- 216-835-0
- EC Name:
- Ethylcyclohexane
- Cas Number:
- 1678-91-7
- Molecular formula:
- C8H16
- IUPAC Name:
- ethylcyclohexane
- Test material form:
- liquid: volatile
Constituent 1
- Specific details on test material used for the study:
- Name Ethylcyclohexane
Appearance colourless transparant liquid
Composition Ethycyclohexane >99%
CAS No. 1678-91-7
EINECS-No. 216-835-0
Molecular formula C8H16
Molecular weight 112 g /mol
Purity >99.9% (GC)
Homogeneity homogeneous
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Specification and Origin
The microbial inoculum to be used in this study consists of activated sludge from a sewage treatment plant treating predominantly domestic sewage.
Origin sewage treatment plant Neustadt/W
Date of collection 1 or 2 days before the start of the test
Preparation
The sludge was filtrated or centrifuged and re-suspended in tap water. The activated sludge
was aerated until usage in the test and fed daily with 50 mL synthetic sewage feed/L.
On the day of the start of the test the dry matter was determined by drying a volume of 5 mL at 105 ± 1 °C and weighing the remnants. The sludge then was adjusted to a suspended solids level of 2.0 – 3.2 g/L with tap water and aerated until usage in the test.
Before the test, pH was checked and adjusted to 6.0 – 8.0.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20.3 – 22.8 °C
- pH:
- 7.7-7.9
- Nominal and measured concentrations:
- 1000 mg/L, 320 mg/, 100 mg/L, 32 mg/L, 10 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- EC50:10 mg/L
Any other information on results incl. tables
O2-Consumption, Inhibition
Vessel No. |
Content |
Concentration in mg/L nominal |
O2consumption in mg/(L*min.) |
O2consumption in mg/(L*h) |
Inhibition in % |
pH |
1 |
Blank Control |
0 |
0.6248 |
37.487 |
0 |
7.9 |
2 |
Blank Control |
0 |
0.6670 |
40.019 |
0 |
7.9 |
3 |
Positive Control |
5 |
0.5668 |
34.009 |
20.3 |
7.8 |
4 |
Positive Control |
10 |
0.3086 |
18.518 |
56.6 |
7.7 |
5 |
Positive Control |
20 |
0.1508 |
9.047 |
78.8 |
7.8 |
6 |
Positive Control |
40 |
0.0783 |
4.699 |
89.0 |
7.9 |
7 |
Blank Control |
0 |
0.7215 |
43.290 |
0 |
7.8 |
8 |
Blank Control |
0 |
0.6769 |
40.613 |
0 |
7.8 |
9 |
Test Item |
1000 |
0.5676 |
34.054 |
20.2 |
7.9 |
10 |
Test Item |
1000 |
0.6068 |
36.409 |
14.7 |
7.9 |
11 |
Test Item |
1000 |
0.6126 |
36.757 |
13.9 |
7.9 |
12 |
Test Item |
1000 |
0.6179 |
37.072 |
13.1 |
7.8 |
13 |
Test Item |
1000 |
0.6220 |
37.321 |
12.5 |
7.9 |
14 |
Test Item |
320 |
0.6530 |
39.179 |
8.2 |
7.9 |
15 |
Test Item |
320 |
0.6656 |
39.938 |
6.4 |
7.9 |
16 |
Test Item |
320 |
0.5680 |
34.079 |
20.1 |
7.9 |
17 |
Test Item |
320 |
0.7036 |
42.215 |
1.1 |
7.9 |
18 |
Test Item |
320 |
0.6872 |
41.231 |
3.4 |
7.9 |
19 |
Test Item |
100 |
0.5742 |
34.450 |
19.3 |
7.9 |
20 |
Test Item |
100 |
0.7812 |
46.870 |
-9.8 |
7.9 |
21 |
Test Item |
100 |
0.6580 |
39.479 |
7.5 |
7.9 |
22 |
Test Item |
100 |
0.5606 |
33.638 |
21.2 |
7.9 |
23 |
Test Item |
100 |
0.6119 |
36.715 |
14.0 |
7.9 |
24 |
Test Item |
32 |
0.7629 |
45.776 |
-7.3 |
7.9 |
25 |
Test Item |
32 |
0.6981 |
41.883 |
1.8 |
7.9 |
26 |
Test Item |
32 |
0.7502 |
45.015 |
-5.5 |
7.9 |
27 |
Test Item |
32 |
0.7487 |
44.924 |
-5.3 |
7.9 |
28 |
Test Item |
32 |
0.6996 |
41.977 |
1.6 |
7.9 |
29 |
Test Item |
10 |
0.7213 |
43.276 |
-1.4 |
7.9 |
30 |
Test Item |
10 |
0.7899 |
47.391 |
-11.1 |
7.9 |
31 |
Test Item |
10 |
0.7949 |
47.691 |
-11.8 |
7.9 |
32 |
Test Item |
10 |
0.8222 |
49.331 |
-15.6 |
7.9 |
33 |
Test Item |
10 |
0.6831 |
40.984 |
3.9 |
7.9 |
34 |
Blank Control |
0 |
0.8290 |
49.737 |
0 |
7.9 |
35 |
Blank Control |
0 |
0.7477 |
44.862 |
0 |
7.9 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition of the respiration of activated sludge when exposed to ethylcyclohexane was determined according to OECD Guideline 209.
Two experiments were performed. In the first experiment 1 of the validity criteria wasn’t met. Therefore, the study was repeated.
In the second experiment the test item was tested using 5 concentrations ranging from 1000 to 10 mg/L nominal concentration. The concentrations based on a non GLP range finding test.
The dry matter of the inoculum was determined as 3.02 g suspended solids/L, giving a concentration of 1.51 g suspended solids/L in the test.
For the positive control an EC50 of 10 mg/L (95% confidence interval: not determinable) was determined. The value lies within the recommended range of the OECD guideline.
The following results for the test item Ethylcyclohexane were determined:
3h NOEC = 320 mg/L
3h EC10 = 300 mg/L
Confidence interval: 150 – 1000 mg/L
3h EC50 > 1000 mg/L
Confidence interval: not determinable - Executive summary:
The inhibition of the respiration of activated sludge when exposed to ethylcyclohexane was determined according to OECD Guideline 209.
Two experiments were performed. In the first experiment 1 of the validity criteria wasn’t met. Therefore, the study was repeated.
In the second experiment the test item was tested using 5 concentrations ranging from 1000 to 10 mg/L nominal concentration. The concentrations based on a non GLP range finding test.
The dry matter of the inoculum was determined as 3.02 g suspended solids/L, giving a concentration of 1.51 g suspended solids/L in the test.
For the positive control an EC50 of 10 mg/L (95% confidence interval: not determinable) was determined. The value lies within the recommended range of the OECD guideline.
The following results for the test item Ethylcyclohexane were determined:
3h NOEC = 320 mg/L
3h EC10 = 300 mg/L
Confidence interval: 150 – 1000 mg/L
3h EC50 > 1000 mg/L
Confidence interval: not determinable
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