Triflumezopyrim

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Quartz sand

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

18.0 to 22.0°C

pH:

Test initiation: 7.52 to 7.59
Test termination: 7.20 to 7.27

Moisture:

Initiation: 24.82 to 25.85% (equivalent to 57.49 to 59.88% of the total water holding capacity)
Termination: 24.09 to 26.13% (equivalent to 55.80 to 60.53% of the total water holding capacity)

Nominal and measured concentrations:

0, 62.5, 125, 250, 500 and 1000 mg/kg dry soil

Reference substance (positive control):

yes

Remarks:

2-Chloroacetamide (tested twice per year)

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Highest dose tested

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

< 62.5 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: biomass

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Highest dose tested

Validity criteria fulfilled:

yes

Conclusions:

7 d and 14 d LC50 (Eisenia fetida): >1000 mg/kg dry soil (mortality)
14 d NOEC (Eisenia fetida): <62.5 mg/kg dry soil (biomass)
14 d NOEC (Eisenia fetida): 1000 mg/kg dry soil (mortality)

Executive summary:

The acute toxicity of the test substance to the earthworm, Eisenia fetida (Savigny), was estimated in a 14-day soil exposure laboratory study conducted as per OECD Guideline 207 and ISO-Guideline 11268-1. Four replicates of ten clitellated adult earthworms were each exposed to nominal concentrations of 62.5, 125, 250, 500 and 1000 mg/kg dry soil. The untreated control (Quartz sand) was replicated four times, with ten earthworms in each replicate.

The toxic reference, 2-Chloroacetamide, is tested twice a year. The 14-day LC50 of the most recent test was 30.8 mg Chloroacetamide/kg dry soil.

Earthworms were assessed for mortality and behavioral effects after 7 and 14 days of exposure and earthworm body weights were assessed at day 0 and day 14.

According to the results of this study, the 7-day and the 14-day LC50 of the test substance was determined to be greater than 1000 mg/kg dry soil. The 14-day No-Observed-Effect Concentration (NOEC) with respect to biomass and mortality was determined to be less than 62.5 mg and 1000 mg/kg dry soil, respectively.

Description of key information

14 -day EC50 (earthworm) > 1000 mg/kg; OECD ; Reliability = 1

14-day NOEC ( Eisenia fetida, earthworm) > 1000 mg/kg (mortality); OECD 207; Reliability = 1

14-day NOEC ( Eisenia fetida, earthworm) < 62.5 mg/kg (biomass); OECD 207; Reliability = 1

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw

Long-term EC10, LC10 or NOEC for soil macroorganisms:

62.5 mg/kg soil dw