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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report Date:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
13 April 2004
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Test solutions

Vehicle:
yes
Details on test solutions:
water (ISO medium)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
pt 5.3 descrizione

Study design

Test type:
static
Water media type:
other: reconstitute water (ISO medium according to OECD 202)
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
249 mg/L (as CaCO3)
Test temperature:
Temperature: The test temperature was in the range of 20.9 – 21.2°C measured in the test vessels during the test. The additionally measured ambient temperature within the climate chamber was between 19.9 – 21.8°C
pH:
The pH of the test solutions was not adjusted and did not vary by more than 1.5 units in any one test. The pH was in the range of 7.37 – 8.00 during the test.
Dissolved oxygen:
Oxygen Concentration: The dissolved oxygen concentration was in the range of 6.64– 8.10 mg/L during the test.
Details on test conditions:
Light-Dark Cycle: The light-dark cycle during the test was 16 hours light (artificial illumination) and 8 hours darkness. The measured light intensity was 723 lux in the climate chamber at the start of the test.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Immobilization of the test animals (detailed table)

Nominal concentration Replicate Number of Number of immobilised animals
[mg/L] treated animals 24 h 48 h

Control 1 5 0 0
2 5 0 0
3 5 0 0
4 5 0 0
100 1 5 0 0
2 5 0 0
3 5 0 0
4 5 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item Acetylglycyrrhetinic acid/Q had no toxic effects on the mobility of the daphnids up to the limit concentration of 100 mg/L.
All validity criteria were met. The results are based on the nominal test item concentration (100 mg/L) and were determined directly from the raw data.