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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1983

Materials and methods

Objective of study:
distribution
Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Distribution of zinc to different organs measured for up to 14 d after single oral administration of radiolabelled zinc chloride to male Wistar rats.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Zinc chloride
- Locations of the label (if radiolabelling): Zn
Radiolabelling:
yes

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 240 g
- Diet: Granulated feed supplied by Bacutil and supplemented with white bred, milk and carrot.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Details on exposure:
No data
Duration and frequency of treatment / exposure:
Single administration
Doses / concentrations
Remarks:
Doses / Concentrations:
0.1 µCi (3.7 kBq) of Zn (65)
No. of animals per sex per dose:
30
Control animals:
other: not applicable
Positive control:
Not applicable
Details on study design:
No data
Details on dosing and sampling:
PHARMACOKINETIC STUDY (Distribution)
- Tissues and body fluids sampled: Stomach, small intestine, large intestine without its contents, liver, kidneys, lungs, spleen, testicle, prostate, brain, blood, heart, thigh muscles and hairs.
- Time and frequency of sampling: After 6 and 24 h and after 2, 4, 7, 14 d from the administration of radioisotope.
- Other: To avoid any losses of Zn (65), radioactivity of 1 g of each sample (1 mL of blood , whole heart, spleen and prostate) was immediately measured.


Statistics:
Student's t test

Results and discussion

Preliminary studies:
Not performed
Main ADME results
Type:
distribution
Results:
Highest accumulation in small intestine, liver, kidneys and large intestine; smaller amount in lungs and spleen; smallest amounts at nearly same level in brain, prostate, heart, blood, skin, hairs and gonads.

Toxicokinetic / pharmacokinetic studies

Details on absorption:
Not measured
Details on distribution in tissues:
Highest accumulation in small intestine, liver, kidneys and large intestine; smaller amount in lungs and spleen; smallest amounts at nearly same level in brain, prostate, heart, blood, skin, hairs and gonads. (See Fig. 1-15 in the reference for details)
Details on excretion:
Not measured

Metabolite characterisation studies

Metabolites identified:
not measured
Details on metabolites:
Not applicable

Any other information on results incl. tables

None

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): other:
In conclusion, zinc was mainly accumulated in small intestine, liver, kidneys and large intestine after single oral administration of zinc chloride to male Wistar rats.
Executive summary:

A study was conducted to determine the distribution of zinc to different organs after oral administration of zinc chloride in rats.

30 male Wistar rats were intubated with 0.1 µCi (3.7 kBq) 65Zn as zinc chloride. Animals were sacrificed after 6 and 24 h and after 2, 4, 7, 14 d of administration and various organs viz. stomach, small intestine, large intestine without its contents, liver, kidneys, spleen, testicle, prostate, brain, blood, heart, thigh muscles and hairs were analysed for zinc concentration.

Zinc was accumulated in the highest amount within the small intestine, liver, kidneys and large intestine and in smaller amounts in lungs and spleen. The smallest amounts were found at nearly same level in brain, prostate, heart, blood, skin, hairs and gonads.

In conclusion, zinc was mainly accumulated in small intestine, liver, kidneys and large intestine after single oral administration of zinc chloride.