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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Version / remarks:
January 22, 2001
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Version / remarks:
March 4, 2016
Deviations:
no
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil
Radiolabelling:
no
Test temperature:
35°C
Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Acquity UPLC system
- Type, material and dimension of analytical (guard) column: Acquity UPLC HSS Cyano, 100mm x 2.1mm i.d., dp = 1.8 um (Waters)
- Detection system: Acquity UPLC TUV detector (Waters)

MOBILE PHASES
- Type: 55/45 (v/v) methanol/buffer
- pH: 7

REFERENCE SUBSTANCES
- Identity: Acetanilide, Monuron, 2,5-Dichloroaniline, Naphthalene, Benzoic acid phenylester, Fenthion, Phenanthrene, 4,4'-DDT
- blank - Formamide

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 mg/L of test solution
- Quantity of reference substances: approx. 1 g/L in methanol or acetonitrile

REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k': yes
- Calculation of retention times: yes
- Determination of the log Koc value: yes
Type:
Koc
Value:
850 dimensionless
pH:
7
Temp.:
35 °C
Type:
log Koc
Value:
2.93 dimensionless
pH:
7
Temp.:
35 °C
Details on results (HPLC method):
The equation of the regression line was: log k’ = 0.329 x log Koc – 0.817 (r = 0.98, n = 16).

Table 1: Koc of the test substance

Substance

Retention time (min)

Log Koc

Koc

tr, 1

tr, 2

mean

Formamide (t0)

0.724

0.723

0.724

 

 

Acetanilide

0.961

0.959

 

1.26

 

Monuron

1.201

1.197

 

1.99

 

2,5-Dichloroaniline

1.389

1.383

 

2.55

 

Naphthalene

1.639

1.631

 

2.75

 

Benzoic acid phenylester

1.964

1.951

 

2.87

 

Fenthion

2.643

2.617

 

3.31

 

Phenanthrene

2.962

2.933

 

4.09

 

4,4’ -DDT

7.510

7.360

 

5.63

 

Test Item

1.735

1.740

1.738

2.93

8.5 x 102

Validity criteria fulfilled:
yes
Conclusions:
The Koc and Log Koc value of the test item was 850 and 2.93 respectively.

Description of key information

OECD guideline, GLP compliant study. HPLC method to determine the Log Koc and Koc values at 35°C.

Koc and Log Koc value was determined to be 850 and 2.93, respectively. This indicates that the substance is of high mobility and not expected to adsorb to soil.

Key value for chemical safety assessment

Koc at 20 °C:
850

Additional information