Registration Dossier
Registration Dossier
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EC number: 276-470-8 | CAS number: 72208-20-9
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Jan - 13 Feb 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- 08/1989
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium dihydrogen phosphate
- EC Number:
- 276-471-3
- EC Name:
- 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium dihydrogen phosphate
- Cas Number:
- 72208-21-0
- Molecular formula:
- C23H26N3.H2O4P
- IUPAC Name:
- 2-(2-{4-[(2-cyanoethyl)(methyl)amino]phenyl}vinyl)-1,3,3-trimethyl-3H-indolium dihydrogen phosphate
- Test material form:
- solid
- Details on test material:
- Basic Red 14 H2PO4
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Inoculum I (Wupperverband): small wastewater treatment plant with domestic sewage
Inoculum II (KA Odenthal): large wwtp with domestic sewage
Inoculum III (GKW Bürrig): predominantly industrial sewage
Day of collection: 16 Jan 1994
Pretreatment: washed twice, separation via centrifugation - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to guideline
- Test temperature: 20-25°C
SAMPLING
- Sampling frequency: 0h, 3h, 7d, 14d, 21d, 28d
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Remarks:
- mean of three tests
- Value:
- 7
- St. dev.:
- 0.47
- Sampling time:
- 28 d
- Details on results:
- Inoculum I: 7% after 28 d
Inoculum II: 7% after 28 d
Inoculum III: 6% after 28 d
All values corrected with blind value
BOD5 / COD results
- Results with reference substance:
- 100% after 14 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The inherent biodegradability of the test item was determined in three inocula of different wastewater treatment plants. Based on the DOC removal after 28 d, the mean biodegradation rate was calculated at 7%. The substance is therefore not considered inherently biodegradable in the OECD 302B test.
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