Essential oil of Matricaria recutita (Asteraceae) obtained from flower tops by steam distillation of European origin

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 23 to 24 November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

GLP compliance programme (inspected on 20-21 June 2017 / signed on 4 September 2017)

Type of method:

differential scanning calorimetry

Key result

Decomposition:

yes

Decomp. temp.:

> 180 °C

Remarks on result:

other: During the heating phase an exothermal effect was observed starting at 180 °C

Results

Boiling point / boiling range : Differential scanning calorimetry

Two DSC measurements in aluminium crucibles with a hole with addition of glass pearls were performed. In each case the test item was cooled down to -50 °C with a cooling rate of 1 K/min, kept at ‑50 °C for 30 min and afterwards the test item was heated up to 500 °C with a heating rate of 10 K/min. No effect was observed during the cooling phase and keeping the test item at -50 °C. During the heating phase an exothermal effect was observed starting at 180 °C, directly followed by an endothermal effect.

The results of the DSC measurements are summarized in the table below:

Table 4.3/1.- DSC measurements

Test item / mg	Starting temperature / °C	Final test temperature / °C	Temperature range / °C	Observations after the measurement
12.69	see above	see above	180 – 270 (exothermal) 270 – 380 (endothermal)
13.39	see above	see above	180 – 270 (exothermal) 270 – 380 (endothermal)	Crucible was empty.

Graphical presentations of the DSC runs are shown on page 7 of the experimental report.

Under consideration of the result from the DSC measurement it was concluded that the test item had no boiling point at atmospheric conditions. Decomposition started at approx. 180 °C.

Conclusions:

The test item had no boiling point before decomposition at atmospheric conditions. Decomposition started at approx. 180 °C .

Executive summary:

The initial boiling point of the test item was measured under GLP according to OECD 103 / EU A2 guideline, by DSC.

Two DSC measurements in aluminium crucibles with a hole with addition of glass pearls were performed. In each case the test item was cooled down to -50 °C with a cooling rate of 1 K/min, kept at -50 °C for 30 min and afterwards the test item was heated up to 500 °C with a heating rate of 10 K/min. During the heating phase an exothermal effect was observed starting at 180 °C, directly followed by an endothermal effect.

Description of key information

The test item had no boiling point at atmospheric conditions before decomposition. Decomposition started at approx. 180 °C

Additional information

A fully reliable experimental study, conducted according to a recognized OECD/EC method and under GLP, is available.

It is considered as a key study, and the result is retained as key data.