Reaction Mass of Didodecyl nonylphenyl phosphite and Dodecyl bis(4-nonylphenyl) phosphite and 4-nonylphenyl ditridecyl phosphite and Bis(4-nonylphenyl) tridecyl

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

84/449/EEC C.2 Acute toxicity for daphnia

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Didodecyl nonylphenyl phosphite

EC Number:

252-457-2

EC Name:

Didodecyl nonylphenyl phosphite

Cas Number:

35239-35-1

Molecular formula:

C39-H73-O3-P

IUPAC Name:

Phosphorous acid, didodecyl nonylphenyl ester

Constituent 2

Reference substance name:

Dodecyl bis(4-nonylphenyl) phosphite

EC Number:

284-117-4

EC Name:

Dodecyl bis(4-nonylphenyl) phosphite

Cas Number:

84787-76-8

Molecular formula:

C42-H71-O3-P

IUPAC Name:

Phosphorous acid, dodecyl bis(4-nonylphenyl) ester

Constituent 3

Reference substance name:

4-nonylphenyl ditridecyl phosphite

EC Number:

284-119-5

EC Name:

4-nonylphenyl ditridecyl phosphite

Cas Number:

84787-77-9

Molecular formula:

C41-H77-O3-P

IUPAC Name:

Phosphorous acid, 4-nonylphenyl ditridecyl ester

Constituent 4

Reference substance name:

Bis(4-nonylphenyl) tridecyl phosphite

EC Number:

284-120-0

EC Name:

Bis(4-nonylphenyl) tridecyl phosphite

Cas Number:

84787-78-0

Molecular formula:

C43-H73-O3-P

IUPAC Name:

Phosphorous acid, bis(4-nonylphenyl) tridecyl ester

impurity 1

Reference substance name:

Tris(nonylphenyl) phosphite

EC Number:

247-759-6

EC Name:

Tris(nonylphenyl) phosphite

Cas Number:

26523-78-4

IUPAC Name:

tris(2-nonylphenyl) phosphite

impurity 2

Reference substance name:

Didodecyl phosphonate

EC Number:

244-325-8

EC Name:

Didodecyl phosphonate

Cas Number:

21302-09-0

Molecular formula:

C24H51O3P

IUPAC Name:

Di(n-dodecyl)hydrogen phosphate

impurity 3

Reference substance name:

Phenol, 4-nonyl-, branched

EC Number:

284-325-5

EC Name:

Phenol, 4-nonyl-, branched

Cas Number:

84852-15-3

Molecular formula:

C15H24O

IUPAC Name:

4-Nonylphenol, branched

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

504 mg test substance and 503 mg DMF/Tween80 (96/4%) were mixed together.

40.9 mg of this Suspension were mixed and made up to 1000 ml with water, achieving aconcentration of 20.5 mg/1.

This solution was used for the preparation of the test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Cultures of daphnia are maintained in glass vessels containing approx. 2.5 1 of reconstituted water at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with • a Suspension of green algae (Scenedesmus subspicatus) supplemented by a Suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.

24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 pm sieve. This Operation is repeated immediately before the exposure and the young (6-24h of age) are retained for the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 1°C

Nominal and measured concentrations:

Nominal : 0.1, 0.18, 0.32, 0.58 and 1.0 mg test substance/L
Actual : 0.1, 0.2, 0.5, 0.8 and 1.6 mg test substance/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.38 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Immobilizations were recorded after 24 and 48 hours exposure

Any other information on results incl. tables

EC 50 (48 h): 95 % confidence limit: 0.31-0.45 mg/L

Applicant's summary and conclusion

Conclusions:

The 48-hr EC50 was 0.38 mg/L.

Executive summary:

The 48-hr EC50 was 0.38 mg/L.