Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
84/449/EEC C.2 Acute toxicity for daphnia
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Didodecyl nonylphenyl phosphite
EC Number:
252-457-2
EC Name:
Didodecyl nonylphenyl phosphite
Cas Number:
35239-35-1
Molecular formula:
C39-H73-O3-P
IUPAC Name:
Phosphorous acid, didodecyl nonylphenyl ester
Constituent 2
Chemical structure
Reference substance name:
Dodecyl bis(4-nonylphenyl) phosphite
EC Number:
284-117-4
EC Name:
Dodecyl bis(4-nonylphenyl) phosphite
Cas Number:
84787-76-8
Molecular formula:
C42-H71-O3-P
IUPAC Name:
Phosphorous acid, dodecyl bis(4-nonylphenyl) ester
Constituent 3
Chemical structure
Reference substance name:
4-nonylphenyl ditridecyl phosphite
EC Number:
284-119-5
EC Name:
4-nonylphenyl ditridecyl phosphite
Cas Number:
84787-77-9
Molecular formula:
C41-H77-O3-P
IUPAC Name:
Phosphorous acid, 4-nonylphenyl ditridecyl ester
Constituent 4
Chemical structure
Reference substance name:
Bis(4-nonylphenyl) tridecyl phosphite
EC Number:
284-120-0
EC Name:
Bis(4-nonylphenyl) tridecyl phosphite
Cas Number:
84787-78-0
Molecular formula:
C43-H73-O3-P
IUPAC Name:
Phosphorous acid, bis(4-nonylphenyl) tridecyl ester
impurity 1
Reference substance name:
Tris(nonylphenyl) phosphite
EC Number:
247-759-6
EC Name:
Tris(nonylphenyl) phosphite
Cas Number:
26523-78-4
IUPAC Name:
tris(2-nonylphenyl) phosphite
impurity 2
Reference substance name:
Didodecyl phosphonate
EC Number:
244-325-8
EC Name:
Didodecyl phosphonate
Cas Number:
21302-09-0
Molecular formula:
C24H51O3P
IUPAC Name:
Di(n-dodecyl)hydrogen phosphate
impurity 3
Chemical structure
Reference substance name:
Phenol, 4-nonyl-, branched
EC Number:
284-325-5
EC Name:
Phenol, 4-nonyl-, branched
Cas Number:
84852-15-3
Molecular formula:
C15H24O
IUPAC Name:
4-Nonylphenol, branched
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
504 mg test substance and 503 mg DMF/Tween80 (96/4%) were mixed together.

40.9 mg of this Suspension were mixed and made up to 1000 ml with water, achieving aconcentration of 20.5 mg/1.

This solution was used for the preparation of the test concentrations.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Cultures of daphnia are maintained in glass vessels containing approx. 2.5 1 of reconstituted water at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with • a Suspension of green algae (Scenedesmus subspicatus) supplemented by a Suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.

24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 pm sieve. This Operation is repeated immediately before the exposure and the young (6-24h of age) are retained for the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 ± 1°C
Nominal and measured concentrations:
Nominal : 0.1, 0.18, 0.32, 0.58 and 1.0 mg test substance/L
Actual : 0.1, 0.2, 0.5, 0.8 and 1.6 mg test substance/L
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.38 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Immobilizations were recorded after 24 and 48 hours exposure

Any other information on results incl. tables

EC 50 (48 h): 95 % confidence limit: 0.31-0.45 mg/L

Applicant's summary and conclusion

Conclusions:
The 48-hr EC50 was 0.38 mg/L.
Executive summary:

The 48-hr EC50 was 0.38 mg/L.