Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
3 Jan 2007 - 5 Jan 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 09898602
- Date received: 6 Nov 2006
- Expiration date of the lot/batch:> 6 months from recepit date at 25°C
- Purity: 99.8%
- Physical Appearance: yellow liquid
- Storage: refrigerated, 1-10°C

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain/clone: Straus
- Source: The Environmental Agency, Portsmouth, UK
- Age of parental stock: 29 days
- Feeding during test: not fed

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48

Test conditions

Hardness:
91 mg/L
Test temperature:
19.5 - 20.0 °C
pH:
7.2 - 7.9
Dissolved oxygen:
99 - 101
Details on test conditions:
TEST SYSTEM
- Test vessel: Petri-dish
- Volume: 125 mL
- Renewal rate of test solution: N/A
- No. of organisms per vessel: 5

OTHER TEST CONDITIONS
- Photoperiod: 16-hor light and 8-hour dark

VEHICLE CONTROL PERFORMED: no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
151 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
62.5 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-hour EC50 and No Observed Effect Concentration (NOEC) values were reported to be 151 mg/L and 62.5 mg/L, respectively.