D-glucitol monolaurate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 - 29 Sep 2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

91884-53-0

IUPAC Name:

91884-53-0

Details on test material:

- Name of test material (as cited in study report): only trade name given
- Composition sorbitansesquioctanat
- Physical state: liquid
- Analytical purity: 99%
- Lot/batch No.: ES 65631498
- Manufacturing date: 2005-06-28
- Shelf life: 36 months
- Stability in solution: stable to at least 150 °C, stable at pH 4 - 14

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
A stock solution of 1 g/L was prepared in dilution water. Each test concentration was prepared in a volumetric flask of 250 mL by dilution of appropiate amount of the stock solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture since May 2001, originally obtained from Landesanstalt für Wasser und Abfallwirtschaft, Düsseldorf, Germany
- Method of breeding: 15 -20 adult organisms were held in one beaker filled with approx. 1400 mL dilution water. The temperature was 20 ± 2 °C and a light-dark circle of 16 to 8 h was kept. The water was renewed three times per week. Scenedesmus subspicatus served as food daily.
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.0 - 20.7 °C

pH:

7.59 - 8.48

Dissolved oxygen:

61 - 79%

Nominal and measured concentrations:

Nominal: 0, 6.25, 12.5, 25, 50 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel
- Type: open
- Material, size, headspace, fill volume: 50 mL glass beakers containing 30 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 6 (one for pysico-chemical measurements only, leaving 5 vessels for effects assessment)
- No. of vessels per control (replicates): 6 (one for pysico-chemical measurements only, leaving 5 vessels for effects assessment)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was prepared separately in a PVC tank by mixing dechlorinated tap water with demineralized water and sulphuric acid. The dilution water was filtered through an active carbon filter.
- Total hardness: 2.376 mmol/L
- Carbonate hardness: 0.700 mmol/L
- pH: 8.02
- Alkalinity: 1.5 mmol/L (determined not in compliance with GLP)
- Ca/Mg ratio: 3.6:1
- Na/K ratio: 7.4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, dissolved oxygen and temperature were measured daily in additional vessels

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilisation recorded after 1 and 2 days

TEST CONCENTRATIONS
- Preliminary study (limit test)
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: A mortality of 68% at 100 mg/L was observed.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

2 d

Dose descriptor:

EC0

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

2 d

Dose descriptor:

EC50

Effect conc.:

39 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 35 - 44 mg/L

Effect concentrations 3

Duration:

2 d

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- The test substance had a negative influence on the swimming behaviour of the test organisms.
- Effect values after 1 day: EC0 = 25 mg/L; EC50 = 65 mg/L; EC100 > 100 mg/L
- Mortality of control: none
- TOC measurements during the preliminary limit test indicated no loss of test substance during the course of the test

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50 (24h): 1.3 mg/L which is within the expected limit of 0.6 - 2.1 mg/L (experiment conducted 20 -21 Sep 2005)

Reported statistics and error estimates:

The EC50 value was calculated with an US EPA computer programm (v 1.5) executing the Trimmed Spearman-Karber Method. The 95% confidence interval was indicated.

Any other information on results incl. tables

Table 1: Immobilisation of Daphnia

Test concentration [mg/L]	Number of immobile daphnia		Cumulative immobile daphnia [%]
	1 d	2d	1 d	2 d
control	0	0	0	0
6.25	0	0	0	0
12.5	0	0	0	0
25	0	0	0	0
50	7	21	28	84
100	21	24	84	96