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EC number: 279-087-4 | CAS number: 79135-87-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- May the 16th,1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- Justification for read across is detailed in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted on 4 April, 1989
- GLP compliance:
- yes
- Details on test solutions:
- The test item was dissolved in bidistilled water at a concentration of 500 mg/l.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- PRETREATMENT
- Name and location of sewage treatment plant where inoculum was collected: ARA, Sissach/Switzerland.
- Incubation: 22 °C; under areation.
- pH (final): 7.8
- rRe-treatment: the sludge was coarsely sieved, centrifuged, the supernatant liquid phase decanted, the solid material resuspended in tap water and again centrifuged. The procedure was repeated a further two times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (ml) to its dry weight (g) determined.
- Preparation of the inoculum: a volume of washed sludge suspension corresponding to 4.0 g dry material were made up with Soerensen buffer (pH= 7) to 1 litre. To that mixture, 50 ml OECD recommended synthetic sewage feed were added. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 22 °C
- Nominal and measured concentrations:
- 3.2, 10, 32, 50 and 100 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks (> 500 ml)
- Aeration: 0.2 l/minute.
- Amount of sludge: 200 ml
MEASUREMENTS
After 180 minutes first sample was taken for oxygen measurement over a period of 10 minutes. Thereafter, in 15 minutes intervals, the rest of the samples were submitted to measurement of oxygen concentration. During measurement the sample was not areated but continuosly stirred. pH was measured along with oxygen measurements.
- Apparatus: WTW OXI-meter and WTW pH-meter - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol sodium salt
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Respiration rate of the sludge was inhibited by 0.7 % at the maximum tested concentration (100 mg/l). Therefore, the test item IC50 is higher than 100 mg/l.
- Results with reference substance (positive control):
- At a concentration of the standard of 10 mg/l the respiration rate was inhibited to 51.7 %. The IC50 determined by linear regression was found to be 7.2 mg/l. Almost complete inhibition (97.2 %) was observed at 50 mg/l.
- Validity criteria fulfilled:
- yes
- Remarks:
- Variation coefficient of oxygen uptake of controls <30 %; inhibitory effects of reference substance within the the 5-30 mg/l IC50 range.
- Conclusions:
- IC50 (3h) > 100 mg/l (nominal)
IC10 (3h) > 100 mg/l (nominal) - Executive summary:
Test article was investigated for inhibitory effects on aerobic waste-water bacteria, according to OECD 209. The test is considered valid since it met all the requirement of the followed guideline.
Up to the maximum tested nominal concentration of 100 mg/l, no adverse effect on microorganisms was observed (only 0.7 % of inhibition), therefore IC50 (3 h) was esteblished to be higher than 100 mg/l.
Conclusion
IC50 (3h) > 100 mg/l (nominal)
IC10 (3h) > 100 mg/l (nominal)
Reference
Influence of test item and reference substance on oxygen consumption
Flask | Chemical | Concentration [mg/l] | Oxygen consumption [mg O2/l min] | Inhibition [%] | Starting pH |
1 | control | - | 0.73 | - | 7.6 |
12 | control | - | 0.72 | - | 7.6 |
7 | test article | 100 | 0.72 | 0.7 | 7.6 |
8 | test article | 50 | 0.73 | -0.7 | 7.6 |
9 | test article | 32 | 0.77 | -6.2 | 7.6 |
10 | test article | 10 | 0.73 | -0.7 | 7.6 |
11 | test article | 3.2 | 0.75 | -3.4 | 7.6 |
2 | positive control | 50 | 0.02 | 97.2 | 7.6 |
3 | positive control | 32 | 0.03 | 95.9 | 7.6 |
4 | positive control | 10 | 0.35 | 51.7 | 7.6 |
5 | positive control | 3.2 | 0.56 | 22.8 | 7.6 |
6 | positive control | 1 | 0.67 | 7.6 | 7.6 |
Description of key information
Non toxic to microorganisms
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
The substance was tested for toxicity potential to microorganisms (activated sludge) following the ETAD recommended method 103. The IC50 was indicted to be higher than 100 mg/l. Unfortunately, a complete test report is not available and only a sheet of test results can currently be retrieved; in addition, the lot tested was characterized by a limited content of Optical Brightener 380. Therefore, a reliability cannot be assigned and the test has been included just for completeness sake.
In order to confirm the toxicity potential to microorganisms of Optical Brightener 380, the available data on the structural analogous Similar Substance 01 have been taken into consideration. The read across approach can be considered reliable and appropriate to investigate the property (details for the approach are included into the IUCLID section 13).
Similar Substance 01 was investigated for inhibitory effects on aerobic waste-water bacteria, according to OECD 209. The test is considered valid since it met all the requirement of the followed guideline. Up to the maximum tested nominal concentration of 100 mg/l, no adverse effect on microorganisms was observed (only 0.7 % of inhibition).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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