4-((3-acetoxypropyl)amino)-2,2-dimethyl-4-oxobutane-1,3-diyl diacetate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 March to 21 November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

yes

Remarks:

An optional toxicity control as suggested by OECD guideline 301F was not performed. The validity of the study is not adversely affected.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-(acetyloxy)-1-{[3-(acetyloxy)propyl]carbamoyl}-2,2-dimethylpropyl acetate

Cas Number:

98133-47-2

Molecular formula:

C15H25NO7

IUPAC Name:

3-(acetyloxy)-1-{[3-(acetyloxy)propyl]carbamoyl}-2,2-dimethylpropyl acetate

Test material form:

liquid: viscous

Specific details on test material used for the study:

- Lot/batch No: 75518
- Expiration date: Sept 19, 2018
- Purity: 95.7%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant (Bois-de-Bay, Satigny, Switzeland) treating predominantly domestic sewage was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

60 d

Initial test substance concentration

Initial conc.:

50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

87

Sampling time:

28 d

Details on results:

The 10-day window criterion is also fulfilled (11% biodegradation on day 4 and 78% on day 14).

The test was extended beyond the standard 28 days. The mean biodegradation after 60 days was 97%.

Panthenyltriacetate did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

BOD5 / COD results

Results with reference substance:

Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified. The repeatability validity criterion (not more than 20% difference between replicates) is fulfilled.

Interpretation of results:

readily biodegradable

Conclusions:

The ready biodegradability of Panthenyltriacetate was determined by the manometric respiration test.

Panthenyltriacetate undergoes 87% biodegradation after 28 days in the test conditions. The 10-day window criterion is also fulfilled (11% biodegradation on day 4 and 78% on day 14).

Panthenyltriacetate did not inhibit the intrinsic respiration of the inoculum at the test concentrations and was therefore considered to be non-toxic to the inoculum at the test concentration.

Thus, Panthenyltriacetate should be regarded as readily biodegradable according to this test.