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EC number: 227-645-2 | CAS number: 5921-65-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 6-phenyl-1,3,5-triazine-2,4-diyldiamine
- EC Number:
- 202-095-6
- EC Name:
- 6-phenyl-1,3,5-triazine-2,4-diyldiamine
- Cas Number:
- 91-76-9
- IUPAC Name:
- 6-phenyl-1,3,5-triazine-2,4-diamine
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
Doses / concentrations
- Remarks:
- Doses / Concentrations:
male mice; 75, 150, 300 mg/kg b.w. |female mice; 50, 100, 200 mg/kg b.w.
Basis:
Results and discussion
Test results
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- This substance was not mutagenic in bacteria [OECD TG 471]. It induced chromosomal aberration in CHL/IU cells with and without an exogenous metabolic activation system even under the soluble concentrations. It also gave a positive response in the human lymphocytes tested [OECD TG 473] and the mouse lymphoma TK assay [OECD TG 476] but only under the insoluble dose levels. The cytogenetic effect observed in in vitro assays however, could not be reproduced in the micronucleus tests in vivo [OECD TG 474].
Any other information on results incl. tables
STATISTICAL RESULTS:
-The highest dose (300 mg/kg for males and 200 mg/kg b.w. for females)
was estimated by five pre-experiments to the suitable since higher
concentrations were lethal. After treatment with the test item the
number of NCEs was not substantially increased as compared to the mean
value of NCEs of the vehicle control thus indicating that this substance
at the indicated concentrations had no cytotoxic effectiveness in the
bone marrow.
There was no biologically and statistically relevant enhancement in the
frequency of the detected micronuclei after administration of the test
item at any dose level or sampling time as compared to vehicle controle.
40 mg/kg b.w. cyclophosphamide administered orally was used as positive
control which showed a substantial increase of induced micronucleus
frequency.
-micronucleus test results
(A)Male animals
Test group Dose Sampling Sampling NCEs per
--- mg/kg b.w. time (hr) micronuclei(%) 2000 PCEs
vehicle 0 24 0.02 1566
BG 75 24 0.08 1562
BG 150 24 0.09 1814
BG 300 24 0.02 1582
CP 40 24 1.11 2033
Vehicle 0 48 0.01 1694
BG 75 48 0.02 1639
BG 150 48 0.08 1795
BG 300 48 0.07 1755
Vehicle 0 72 0.03 1610
BG 75 72 0.04 1417
BG 150 72 0.03 1549
BG 300 72 0.02 1645
(B)Female animals
Test group Dose Sampling Sampling NCEs per
--- mg/kg b.w. time (hr) micronuclei(%) 2000 PCEs
vehicle 0 24 0.03 1652
BG 50 24 0.03 1563
BG 100 24 0.09 1463
BG 200 24 0.04 1889
CP 40 24 0.98 1755
Vehicle 0 48 0.01 2113
BG 50 48 0.04 1727
BG 100 48 0.01 1628
BG 200 48 0.02 1945
Vehicle 0 72 0.02 1577
BG 50 72 0.02 1408
BG 100 72 0.04 1638
BG 200 72 0.01 1492
BG = benzoguanamine (2,4-diamino-6-phenyl-1,3,5-triazine)
CP = cyclo phosphamide
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
not genotoxic in vivo - Executive summary:
In a CD-1 mouse bone marrow micronucleus assay, 5 male and female were treated oral gavage with this substance at doses of 0, 75, 150, 300 mg/kg bw in male mice and 0, 50, 100, 200 mg/kg/bw. Bone marrow cells were harvested at 24, 48, 72 hr post-treatment. The vehicle was corn oil.
There were no signs of toxicity during the study.The positive control induced the appropriate response. There was not a significant increase in the frequency of micronucleated polychromatic erythrocytes in bone marrow after any treatment time.
This study satisfiesthe requirement for Test Guideline OECD 474 for in vivo cytogenetic mutagenicity data.
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