2-Propenoic acid, 2-methyl-, C11-14-isoalkyl esters, C13-rich

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10-07-1989 - 14-07-1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Nominal test concentration above water solubility.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Test was performed above water solubility of the test substance

GLP compliance:

yes

Analytical monitoring:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Specification: Zebra fish (Brachydanio rerio) HAMILTON-BUCHANAN, Teleostei, Cyprinidae.
Source: German bred; Distributor: Doller, Zierfischzüchterei,Finnentrop-Serkenrode.
Size: 3.0 ± 0.5 cm.
Holding: The test organisms were acclimatised for at least 12 days before start of the test in dilution water of the
same quality as used for the test. The fish were fed daily with TetraMin® Hauptfutter until 24 h before the test.
Mortality: The criteria of the guideline OECD 203 were applied.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 °C ± 1 °C

pH:

7.8 ± 0.2

Dissolved oxygen:

7.88 mg/l

Nominal and measured concentrations:

0 (blank), 1.0, 10,
100, 1000 , 10000 mg/l.

Details on test conditions:

Dilution water was prepared according to the test guideline (OECD 203) following internal SOP’s.

Test concentrations:
Aliquots of the stock solution were mixed with dilution water. The volume of the test solution was adjusted to 10 l each.


Test priciples and procedure:

10 fish were used for each test concentration including controls.
Only fish in good health and free from any apparent malformation were used.


Numbered 10-l glass aquaria were used as test vessels and Filled with 5 l of test-solution. The test animals were added to the previously prepared test solution; controls were kept in dilution water (blank).

The aquaria were slightly aerated via a glass capillary throughout the test.


The fish were exposed to various concentrations of the test substance for a period of 96 hours under static conditions. Visible abnormalities
observed during the first 6 hours of the experiment were recorded. Mortalities and visual abnormalities were recorded at 24 h, 48 h, 72 h, and 96 h.

The test temperature was 23° C ± l °C. The pH of the dilution water was 7.8 ± 0.2.
The light/dark rhythm was 14h/l0h.

The pH and the oxygen concentrations of the control and of all the test concentrations were measured daily.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Concentration-effect relationship Isotridecyl methacrylate

	48 h	96 h
LC0(mg/l) LC50(mg/l) LC100(mg/l) 95% confidence limits (LC50, mg/l) slope chi-square	> 10000 > 10000 > 10000 ---   --- ---	> 10000 > 10000 > 10000 ---   --- ---

Validity criteria fulfilled:

yes

Remarks:

with restrictions (water solubility)

Conclusions:

According to the presented results, the test substance isotridecyl methacrylate is classified as non-toxic to fish (Danio rerio).

Executive summary:

In a 96 h fish acute toxicity test (OECD 203, GLP) Zebra fish (Danio rerio) were exposed to isotridecyl methacrylate at nominal concentrations of 1.0, 10, 100, 1000 and 10000 mg/l. No mortalities were observed, the LC0 value was > 10000 mg/l.

COMMENT: Concentrations used in the test are clearly above the water solubility of isotridecyl methacrylate. Despite the fact that the test therefore has to be considered as limited in validity from a technical point of view, it can be concluded that saturated solutions of the substance are non-toxic to fish.

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

An OECD 203 guideline GLP study with nominal test concentrations above 10000 mg/L showed no toxicity to Danio rerio.
Therefore, it appears that saturated solutions of Isotridecyl methacrylate are non-toxic to fish. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10 000 mg/L

Additional information

LC50 [96 h] > 10000 mg / L

Concentrations used in the available test are five orders of magnitude above the water solubility of Isotridecyl methacrylate, which is < 1 µg/L. Therefore, emulsions or even two-layer systems of the test item with water have been tested instead of solutions setting a limitation to the validity of the study. However, it appears that saturated solutions of the substance are non-toxic to fish.