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Biodegradation in water

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonate can be expected to be range from 0 to 12.807%, respectively in 28 days. Thus, based on this, test chemical tetrasodium 2 -[[4 -[[4 -[(4 -amino-6 -chloro-1,3,5 -triazin-2 -yl)amino]-5 -sulphonato-1 -naphthyl]azo]-7 -sulphonato-1 -naphthyl]azo]benzene-1,4-disulphonate is considered to be not readily biodegradable in nature.

Additional information

Biodegradation in water

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the biodegradability of the test chemical tetrasodium 2 -[[4 -[[4 -[(4 -amino-6 -chloro-1,3,5 -triazin-2 -yl)amino]-5 -sulphonato-1 -naphthyl]azo]-7 -sulphonato-1 -naphthyl]azo]benzene-1,4-disulphonate. The studies are as mentioned below:

28-days Manometric respirometry test following the OECD guideline 301F to determine the ready biodegradability of the test item. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was 30 mg/L, while that of inoculum was 10 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was determined to be 74.074%. The mean BOD values(mg O2/l) in control on 28th day was 9 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 50.050 %after 7 days and 68.068% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid.The BOD28 value of test chemical was observed to be 0.050mgO2sup>/mg. ThOD was calculated as 1.088 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 4.596%. Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable at 20 ± 1°C over a period of 28 days.

In an another 28-days Manometric respirometry test, the ready biodegradability of the test chemical was determined following the OECD guideline 301F. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was 30 mg/L, while that of inoculum was 10 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was determined to be 74.04%. The mean BOD values(mg O2/l) in control on 28th day was 9mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 48.04%after 7 days and 70.07% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid.The BOD28 value of test chemical was observed to be 0.188 mgO2sup>/mg. ThOD was calculated as 1.464 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 12.807 %.Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable at 20± 1°C over a period of 28 days.

Additional biodegradation study was conducted for 5 days for evaluating the percentage biodegradability of test substance under aerobic conditions. Sewage was used as a test inoculum for the study. The percentage degradation of test substance was determined to be 0% by using BOD parameter in 5 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in water.

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonate can be expected to be range from 0 to 12.807%, respectively in 28 days. Thus, based on this, test chemical tetrasodium 2 -[[4 -[[4 -[(4 -amino-6 -chloro-1,3,5 -triazin-2 -yl)amino]-5 -sulphonato-1 -naphthyl]azo]-7 -sulphonato-1 -naphthyl]azo]benzene-1,4 -disulphonate is considered to be not readily biodegradable in nature.