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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
guideline study according to GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction product of 2-hydroxyethylacrylate with imidazole
EC Number:
926-135-3
Cas Number:
1181222-25-2
Molecular formula:
not applicable
IUPAC Name:
Reaction product of 2-hydroxyethylacrylate with imidazole
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: batch: 0015609877
- Expiration date of the lot/batch: Expiry date: 18 Aug 2017
- Purity test date:


Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
3 h

Test conditions

Details on test conditions:
Incubation time: 3 hours
Test temperature: 18.9 – 20.8 °C
Test vessels: Glas-beakers (nominal volume 1 L)
Test volume: 500 mL
Synthetic medium: 16 mL/test vessel of 100-fold concentrated OECD
medium
Oxygen concentration during aeration: > 2 mg/L
Oxygen concentration immediately
before measurement:
> 7 mg/L
Duration of the measurement of
oxygen consumption:
About 8 - 10 min
Sludge concentration in the test vessel: 1.5 g/L Dw

Test system: municipal Activated sludge
Reason for the selection of the test system: required by the test guidelines
Age: 1 day
Origin: aeration tank of the wastewater treatment plant of Mannheim, Germany
Collection of the test system: 25 Jan 2017
Arrival in the test facility: 25 Jan 2017
After arrival of the activated sludge suspension in the test facility the suspension was sieved
with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over
night at room temperature. At the next day the sludge suspension was washed once with
drinking water and the suspension was adjusted to 3 g/L Dw.
3.3. TEST GROUPS AND CONCENTRATIONS
Test concentrations: 1000, 500, 250, 125, 62.5 mg/L as nominal concentration
based on test substance without correction of purity and blank
controls.
100, 10, 1 mg/L as nominal concentration based on reference
substance.
Test replicates: 6 replicates for the control,
3 replicates for each test substance concentration,
2 replicates for each reference substance concentration.
Reason for selection of
test concentrations:
According to the test guidelines, at least 5 concentrations in a
geometric series with a separation factor of ≤ 3.2 should be
used, preferably encompassing the range from 10 – 80%
inhibition .

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The test substance was tested in an OECD 209 guiodelien test for toxicity again microorganisms. The EC10 and EC50 were monitored to be > 1000 mg/l.

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