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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017-01-11 - 2017-02-09
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
new OECD guideline study according to GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction product of 2-hydroxyethylacrylate with imidazole
EC Number:
926-135-3
Cas Number:
1181222-25-2
Molecular formula:
not applicable
IUPAC Name:
Reaction product of 2-hydroxyethylacrylate with imidazole

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany.
The inoculum was collected on 11 January 2017 from the aeration tank of the plant. A
suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh
with a mesh size about 1 mm
Duration of test (contact time):
28 d
Details on study design:
The Carbon Dioxide Evolution Test was performed in 2 L incubation bottles filled up to a volume of 1.5 L. The bottles were connected to two serial scrubbing bottles (total volume 250 mL) filled with 100 mL 0.05 mol sodium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. Usually twice a week the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined and used for the calculation of the produced carbon dioxide. After each sampling the second trap was moved forward and the new trap with fresh sodium hydroxide solution was placed into the second position. Each trap was analyzed separately.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Key result
Parameter:
% degradation (CO2 evolution)
Value:
25
Sampling time:
28 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
13
Sampling time:
5 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
25
Sampling time:
21 d

BOD5 / COD results

Results with reference substance:
Degree of biodegradation of the reference substance after 14 days: 82 % CO2/ThCO2

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable, not fulfilling specific criteria
Executive summary:

The test item 2-Propenoic acid, 2-hydroxyethyl ester, reaction products with 1H-imidazole CAS: 1181222-25-2 is tested for readily biodegradation in an OECD 301 B test. After 28d teh degradation rate reached 25 %.

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