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Toxicity to aquatic algae and cyanobacteria

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Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data from reliable source (NITE (National Institute of Technology and Evaluation) Japan). No test report is available however dat from this source are known to be well documented and the test are performed according to GLP and OECD guidelines.
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Reference substance (positive control):
not specified
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
500 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Validity criteria fulfilled:
not specified
Conclusions:
An OECD 201 static test using Pseudokirchneriella subcapitata derived a 72 hour EC50 of 500 mg/L
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
2017-2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Identification: Hydroxypropyl, 2-, trimethylammonium formate
Physical state/Appearance: Colorless to very light blue liquid
Batch: Chernil.20160628.B
CAS#: 62314-25-4
Purity: 92.25% active in water
Expiry Date: 28 June 2017
Storage Conditions: Room temperature in the dark from 24 October 2016 to
08 November 2016, thereafter, room temperature in the
dark over silica gel
Analytical monitoring:
yes
Details on sampling:
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.
Vehicle:
no
Details on test solutions:
A 1.0 mg/L test solution was prepared from which two samples were taken, one sample was taken for 0-Hour analysis (stored frozen), the other sample was incubated under test conditions for 72 hours prior to analysis. A further aliquot of the initial 1.0 mg/L test
solution was inoculated with algal suspension (nominal cell density 5.00 x 103 cells/mL) from which two samples were taken, one sample was taken for 0-Hour analysis (stored frozen), the other sample was incubated under test conditions for 72 hours prior to analysis. After 72 hours all samples were removed from the incubator and freezer and sent for analysis.
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
The test was carried out using Pseudokirchneriella subcapitata strain CCAP 278/4. Liquid cultures of Pseudokirchneriella subcapitata were obtained from the Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland. Master cultures were maintained in the laboratory by the periodic replenishment of culture medium (Section 3.3). The master cultures were maintained in the laboratory under constant aeration and illumination at 21 ± 2 °C.
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
pH:
7.2 at 0h
7.6 at 72h
Nominal and measured concentrations:
Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to be near nominal with the exception of the 1.0 mg/L test preparations which showed a measured test concentration of 1.8 mg/L at both 0 and 72 hours.
Reference substance (positive control):
yes
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Results with reference substance (positive control):
The results from the positive control with potassium dichromate were within the normal ranges for this reference item.
Validity criteria fulfilled:
yes
Conclusions:
The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period and gave aGrowth Rate EC50 of >100mg/L, a NOEC of 3.2 mg/L and a LOEC of 10 mg/L
It was not possible to calculate an ErC50 value as no concentration tested resulted in 50% inhibition of growth rate.

Description of key information

The effect of the read-across substance TMR2 (Hydroxypropyl, 2-, trimethylammonium formate) on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period in an OECD 201 static test and gave aGrowth Rate EC50 of >100mg/L, a NOEC of 3.2 mg/L and a LOEC of 10 mg/L

It was not possible to calculate an ErC50 value as no concentration tested resulted in 50% inhibition of growth rate.

An OECD 201 static test in Pseudokirchneriella subcapitata using the dissociation product 2-ethylhexanoic acid derived a 72 hour EC50 of 500 mg/L

On this basis the lowest EC50 value of of 100 mg/L. is assigned to TMR (2-hydroxypropyl)trimethylammonium 2-ethylhexanoate)

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L

Additional information