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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Description of key information

The ready biodegradability of the test item was determined with non-adapted activated domestic sludge according to OECD guideline 301 B (CO2evolution test) for a period of 28 days.The study was conducted at Hydrotox GmbH, Labor für Ökotoxikologie und Gewässerschutz, Bötziger Str. 29, 79111 Freiburg, Deutschalnd.

The CO2-evolution test determines the ready biodegradability of testitemsaccording totheModified Sturm test. A solution or suspension of the test item in a mineral medium,corresponding to10-20mgTOC/L, is inoculated with activated sludge (30mgd.s./L).

The test vessels are aerated by the passage of carbon dioxide-free air and are incubatedunderaerobic conditions in diffuse light for 28 days. Degradation is followed by determining the carbon dioxide produced and absorbed to sodium hydroxide via IC-measurement (IC=inorganic carbon). The amount of carbon dioxide produced from the test item less the amount derived from the blank inoculum is expressed as a percentage of ThC02       (theoretical amount of CO2).

 

The biodegradation of the test item showed a high variability and was between 10.3 - 42.3% within 28 days (after acidification). The test item did not reach the criterion of ready biodegradation

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information