(2-hydroxypropyl)trimethylammonium formate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

Minor: During both the range-finding and definitive tests the temperature range exceed the maximum deviation of ± 1 °C

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification: Hydroxypropyl, 2-, trimethylammonium formate
Physical state/Appearance: Clear colorless liquid*
Batch Number: Chernil.20160628.B
CAS#: 62314-25-4
Purity: 95.25% active in water
Expiry Date: 28 June 2017
Storage Conditions: Room temperature in the dark over silica gel

Analytical monitoring:

yes

Details on sampling:

A sample of each test concentration was taken for chemical analysis at 0 and 48 hours in order to determine the stability of the test item under test conditions.
Samples were taken from the control and the 100 mg/L test group from the bulk test preparation at 0 hours and from the pooled replicates at 48 hours for quantitative analysis.
All samples were stored frozen prior to analysis.

Vehicle:

no

Details on test solutions:

At the start and throughout the test the control and the 100 mg/L test solution were observed
to be a clear, colorless solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test was carried out using first instar Daphnia magna derived from in-house laboratory cultures.
Adult daphnids were maintained in 150 mL glass beakers containing Elendt M7 medium in a temperature controlled room maintaining the water temperature at 18 °C to 22 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle
with 20 minute dawn and dusk transition periods. Each culture was fed daily with a mixture of algal suspension (Desmodesmus subspicatus) and Tetramin® flake food suspension.
Culture conditions ensured that reproduction was by parthenogenesis.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours.

pH:

7.7-7.8

Nominal and measured concentrations:

Nominal concentration was 100 mg/L
Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 97% to 99% of the nominal concentration, and so the results are based on the nominal test concentration.

Reference substance (positive control):

yes

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

There was no immobilization in 20 daphnids exposed to a test concentration of 100 mg/L for a period of 48 hours.
No sub-lethal effects of exposure were observed throughout the test.

Results with reference substance (positive control):

The results from the positive control with potassium dichromate were within the normal range for this reference item.

Reported statistics and error estimates:

An estimate of the EC50 values was given by inspection of the immobilization data.

Validity criteria fulfilled:

yes

Conclusions:

Exposure of Daphnia magna to the test item gave EC50 values of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.

Executive summary:

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing ofChemicals (April 2004) No 202, "Daphniasp., Acute Immobilisation Test" referenced asMethod C.2 of Commission Regulation (EC) No. 440/2008.

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) wereexposed to an aqueous solution of the test item at a nominal concentration of 100 mg/L for48 hours at a temperature of 19 °C to 22 °C under static test conditions. Immobilization andany adverse reactions to exposure were recorded after 24 and 48 hours.

Analysis of the test preparations at 0 and 48 hours showed measured test concentrations torange from 97% to 99% of the nominal concentration, and so the results are based on thenominal test concentration.Exposure of Daphnia magnato the test item gave EC50values of greater than 100 mg/L. TheNo Observed Effect Concentration was 100 mg/L.

Description of key information

Exposure of Daphnia magna to the test item gave EC50 values of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L