Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
not specified
Remarks:
Testing done in China. Not specified.
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
Activated Sludge was derived from the DaTanSha Sewage Treatment Plant of Guangzhou. On return to the laboratory
the sludge was washed with tap water. After centrifuging the supernatant was decanted. This procedure was repeated three
times. A small amount of the washed sludge was weighed and dried. From the sludge concentration of 13. lg/L, the amount
of sludge was calculated which was suspended in water in order to obtain an activated sludge with a mixed liquor
suspended solids level of 4g/L and pH was 7-8.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h
Test temperature:
20"C±2"C
Nominal and measured concentrations:
100 mg/l
Reference substance (positive control):
yes
Remarks:
3, 5-dichlorophenol
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
7.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
3, 5-dichloropheno
Basis for effect:
inhibition of total respiration
Remarks on result:
other: Positive control
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
Results
The 3h-EC50 of 3, 5-dichlorophenol was 7.6 mg/Lin range of 5 mg/L-30 mg/L.
According to the result of the range-finding test, the Inhibition rate of the test solution with the highest concentration of
100mg/L was 9.77% so that the 3h-EC50 oftest substance was > 100mg/L
Reported statistics and error estimates:
The two control respiration rates were within 15 percent of each other.
Conclusions:
The Inhibition rate of the test solution with the highest concentration of 100mg/L was 9.77% so that the 3h-EC10 of test substance was > 100mg/L

Description of key information

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information