p-anisic acid

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the analogue justification attached to IUCLID section 13.

Reason / purpose for cross-reference:

other:

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

102.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Result of toxicity control from ready biodegradability test

Validity criteria fulfilled:

yes

Conclusions:

Test item is not inhibitory to aerobic activated sludge microorganisms since degradation was 30% after 14 days (and 46% after 28 d).

Executive summary:

Since no studies on the toxicity to aquatic microorganisms are available for p-anisic acid (CAS 100-09-4) a ready biodegradability study is used to derive a NOEC for the toxicity to aquatic microorganisms. If a compound degrades well in a ready biodegradability test, or does not inhibit the degradation of a positive control at a certain concentration, this concentration can be used as a NOEC value. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).

A substance can be assumed to be not inhibitory to aquatic microorganisms, if in the toxicity control of a ready biodegradation test, more than 25% degradation based on ThOD occurred within 14 days (OECD guideline 301F). For the test item a biodegradation test according to OECD guideline 301F is available (Hammesfahr, 2017). The test includes a toxicity control, which contains 102.7 mg/L of the test item and 104.1 mg/L mg/L of sodium benzoate. The toxicity control attained 30% degradation after 14 days of incubation. Hence, the substance is not toxic to aquatic microorganisms in the toxicity control and the test item concentration of 102.7 mg/L can be used as NOEC.

Description of key information

NOEC (14 d) = 102.7 mg/L (test material, OECD 301F, nominal)

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

102.7 mg/L

Additional information

No data are available on the toxicity to microorganisms of p-anisic-acid (CAS No. 100-09-4). Therefore data from the analogue substance sodium anisate (CAS 536-45-8) were used for read across. This read-across is in accordance with Regulation (EC) No. 1907/2006 Annex XI, 1.5. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment. This read-across is justified in within the analogue justification in IUCLID Section 13.

Since no studies on the toxicity to aquatic microorganisms are available a ready biodegradability study is used to derive a NOEC for the toxicity to aquatic microorganisms. If a compound degrades well in a ready biodegradability test, or does not inhibit the degradation of a positive control at a certain concentration, this concentration can be used as a NOEC value. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).

A substance can be assumed to be not inhibitory to aquatic microorganisms, if in the toxicity control of a ready biodegradation test, more than 25% degradation based on ThOD occurred within 14 days (OECD guideline 301F). For the test item a biodegradation test according to OECD guideline 301F is available. The test includes a toxicity control, which contains 102.7 mg/L of the test item and 104.1 mg/L mg/L of sodium benzoate. The toxicity control attained 30% degradation after 14 days of incubation. Hence, the substance is not toxic to aquatic microorganisms in the toxicity control and the test item concentration of 102.7 mg/L can be used as NOEC.