Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-01-29 to 1990-02-02
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
sampling at start of the test and at the time of 100% mortality or test end of respective test concentration

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test solutions were directly prepared

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- scientific name: Brachydanio rerio Hamilton-Buchanan
- Source: West Aquarium, Bad Lauterberg

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21.0 - 22.3°C
pH:
7.3 - 8.0
Dissolved oxygen:
6.5 - 8.4 mg/L
Nominal and measured concentrations:
Nominal: 0 (control), 18.0, 32.4, 58.3, 105, 189 mg/L
Measured (geometric mean):
Details on test conditions:
Water quality criteria according to guideline
Light regime: 12/12 h
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
32.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
105 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 58.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
geometric mean LC0/LC100
Details on results:
- Behavioural abnormalities: none
- Other biological observations: dye accumulation on skin surface
- Mortality of control: 10%

Any other information on results incl. tables

concentration
[mg/L]
% mortality after
24h 48h 72h 96h
control 0 0 10 10
189.0 100  -  -  -
105.0 50 100  -  -
58.3 10 20 30 30
32.4 0 0 0 0
18.0 0 0 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96-h LC50 to Danio rerio was calculated to be 58.3mg test substance/L. The mortality at 58.3 mg/L is 30%, so the true LC50 is >= 58.3 mg/L. 58.3 mg/L is considered the worst case. The substance is acutely harmful to fish.