1,3,3-trimethyl-2-[(methylphenylhydrazono)methyl]-3H-indolium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

27.-29.07.1993

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-[[(4-methoxyphenyl)methylhydrazono]methyl]-1,3,3-trimethyl-3H-indolium methyl sulphate

EC Number:

258-946-7

EC Name:

2-[[(4-methoxyphenyl)methylhydrazono]methyl]-1,3,3-trimethyl-3H-indolium methyl sulphate

Cas Number:

54060-92-3

Molecular formula:

C20H24N3O.CH3O4S C21H27N3O5S

IUPAC Name:

2-{[2-(4-methoxyphenyl)-2-methylhydrazin-1-ylidene]methyl}-1,3,3-trimethyl-3H-indol-1-ium methyl sulfate

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dilution from stock solution

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

No data

Test temperature:

21.3 - 22 °C

pH:

8.0 - 8.1

Dissolved oxygen:

97 - 99 %

Nominal and measured concentrations:

Nominal: 0 (blank control), 0.01, 0.10, 1.0, 10.0 and 100 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.316 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: at 0.1 mg/L no immobile daphnia after 48h; at 1.0 mg/L 10 (of 10) Daphnia immobile after 48 h

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 of the test item to daphnia is 0.32 mg/L.

Executive summary:

The 48–hr-acute toxicity of the test item to Daphnia was studied at nominal concentrations of 0 (blank control), 0.01, 0.10, 1.0, 10, and 100 mg/L. The 48– hour EC₅₀was determined to be 0.3162 mg/L.