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EC number: 935-977-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
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- Additional physico-chemical properties of nanomaterials
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- Nanomaterial specific surface area
- Nanomaterial Zeta potential
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- Endpoint summary
- Stability
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- Ecotoxicological Summary
- Aquatic toxicity
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- Short-term toxicity to fish
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Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2009-01-14 to 2009-02-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes
- Type of method:
- HPLC estimation method
- Radiolabelling:
- no
- Test temperature:
- 25 °C
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus: HPLC System Agilent 1100 series
- Type, material and dimension of analytical column: LiChrospher 100 CN-5 µm, column size: 250 mm x 0 4.6 mm, particle size: 5 µm, pore size: 100 A, column temperature: 25 °C, material: stainless steel
- Detection system: UV/Vis detector
MOBILE PHASES
- Type: Methanol, distilled water 70:30 % (v/v) (pH= 6.6 at 20.3 °C), isocratic elution
- Experiments with additives carried out on separate columns: no
- Solutes for dissolving test and reference substances: methanol
DETERMINATION OF DEAD TIME
- Method: by using a "dead time marker"
REFERENCE SUBSTANCES
- Identity: Acetanilide, Atrazin, Monuron, Triadimenol, Linuron, Pyrazophos, Diclofop-methyl, DDT
REPETITIONS
- Number of determinations: 2 - Key result
- Type:
- log Koc
- Value:
- 8.31 dimensionless
- Temp.:
- 25 °C
- Key result
- Type:
- Koc
- Value:
- 204 173 795 L/kg
- Temp.:
- 25 °C
- Details on results (HPLC method):
- - Dead time: t0 = 3.30 min
- Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient of the test item was found to be log Koc > 5.6 (8.31) at 25 °C. The Koc was 204173795 L/kg at 25 °C.
- Executive summary:
A study according to OECD 121 and EU Method C.19 was performed to determine the adsorption coefficient log Koc of the test item. The HPLC method was used. A series of reference substance, in accordance to OECD 121, was used as calibration series for the HPLC measurements. Two series were injected. Each series included the calibration substances and the test item. The test item was injected in-between the series of the calibration substances. The dead time t0 was determined by measuring the retention time of a dead time marker - Formamide. The dead time marker was dissolved in methanol and diluted from 0.1 mL to 1 mL with the mobile phase methanol/distilled water 70:30 % (v/v) and injected as single standards twice. The calibration substances were dissolved methanol and diluted from 0.1 mL to 1 mL with the mobile phase methanol/distilled water 70:30 % (v/v) and injected as single standards twice. The test item was dissolved in methanol and diluted from 0.05 mL to 1 mL with 0.65 mL methanol and 0.3 mL distilled water and injected as single sample twice. The dead time t0 was determined to be 3.3 min. The adsorption coefficient of the test item was found to be log Koc > 5.6 (8.31) at 25 °C. The Koc was 204173795 L/kg at 25 °C.
Reference
Table 1: Determination of the Dead Time t0
Dead time marker |
t0 in min 1st Measurement |
t0 in min 2nd Measurement |
t0 in min Mean |
Formamide |
3.30 |
3.30 |
3.30 |
Dead time: t0 = 3.30 min
Table 2: Calibration Data – 1st Measurement (*from OECD guideline 121, adopted January 2001)
Calibration substance |
1st Inj. tR in min |
k |
log k |
log Koc * |
Acetanilide |
3.62 |
0.10 |
-1.01 |
1.25 |
Atrazin |
3.65 |
0.11 |
-0.97 |
1.81 |
Monuron |
3.79 |
0.15 |
-0.82 |
1.99 |
Triadimenol |
3.86 |
0.17 |
-0.77 |
2.40 |
Linuron |
4.08 |
0.24 |
-0.62 |
2.59 |
Pyrazophos |
4.41 |
0.34 |
-0.47 |
3.65 |
Diclofop-methyl |
4.50 |
0.37 |
-0.44 |
4.20 |
DDT |
5.32 |
0.61 |
-0.21 |
5.63 |
Regression: log Koc = a + b * log k
Parameters: a = 6.31
b = 5.08
r2 = 0.9484
Table 3: log Koc of the Test Item – 1st Measurement
|
1st Inj. tR in min |
k |
log k |
log Koc |
Test Item |
11.2 |
2.40 |
0.38 |
>5.61) (8.25) |
1) above log Koc value of the highest calibration substance (DDT)
Table 4: Calibration Data – 2nd Measurement (*from OECD guideline 121, adopted January 2001)
Calibration substance |
2nd Inj. tR in min |
k |
log k |
log Koc * |
Acetanilide |
3.63 |
0.10 |
-0.99 |
1.25 |
Atrazin |
3.66 |
0.11 |
-0.96 |
1.81 |
Monuron |
3.80 |
0.15 |
-0.82 |
1.99 |
Triadimenol |
3.86 |
0.17 |
-0.76 |
2.40 |
Linuron |
4.09 |
0.24 |
-0.62 |
2.59 |
Pyrazophos |
4.42 |
0.34 |
-0.47 |
3.65 |
Diclofop-methyl |
4.50 |
0.37 |
-0.44 |
4.20 |
DDT |
5.32 |
0.62 |
-0.21 |
5.63 |
Regression: log Koc = a + b * log k
Parameters: a = 6.34
b = 5.16
r2 = 0.9498
Table 5: log Koc of the Test Item – 2nd Measurement
|
2nd Inj. tR in min |
k |
log k |
log Koc |
Test Item |
11.4 |
2.47 |
0.39 |
>5.61) (8.37) |
1) above log Koc value of the highest calibration substance (DDT)
Table 6: log Koc of the Test Item
|
log Koc |
1st Measurement |
>5.6 (8.25) |
2nd Measurement |
>5.6 (8.37) |
Mean |
>5.6 (8.31) |
Description of key information
The adsorption coefficient of the test item was found to be log Koc > 5.6 (8.31) at 25 °C. The Koc was 204173795 L/kg at 25 °C (reference 5.4.1-1).
Key value for chemical safety assessment
- Koc at 20 °C:
- 204 173 795
Additional information
A study according to OECD 121 and EU Method C.19 was performed to determine the adsorption coefficient log Koc of the test item. The HPLC method was used. A series of reference substance, in accordance to OECD 121, was used as calibration series for the HPLC measurements. Two series were injected. Each series included the calibration substances and the test item. The test item was injected in-between the series of the calibration substances. The dead time t0 was determined by measuring the retention time of a dead time marker - Formamide. The dead time marker was dissolved in methanol and diluted from 0.1 mL to 1 mL with the mobile phase methanol/distilled water 70:30 % (v/v) and injected as single standards twice. The calibration substances were dissolved methanol and diluted from 0.1 mL to 1 mL with the mobile phase methanol/distilled water 70:30 % (v/v) and injected as single standards twice. The test item was dissolved in methanol and diluted from 0.05 mL to 1 mL with 0.65 mL methanol and 0.3 mL distilled water and injected as single sample twice. The dead time t0 was determined to be 3.3 min. The adsorption coefficient of the test item was found to be log Koc > 5.6 (8.31) at 25 °C. The Koc was 204173795 L/kg at 25 °C (reference 5.4.1-1).
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