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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
8 March 2004 and 5 April 2004.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Version / remarks:
1992
Deviations:
no
Remarks:
The test material rapidly hydrolyses in aqueous media and some of the hydrolysis products may be volatile, so the Manometric Respirometry Test was considered best.
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic, non-adapted
Details on inoculum:
A mixed population of sewage sludge micro-organisms was obtained from the final effluent stage of the Severn Trent Water PLC sewage treatment plant, which treats predominantly domestic sewage. The sample of effluent was filtered through course filter paper and maintained on aeration in a temperature controlled room at 21 °C.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
As biodegradation progresses, the micro-organisms convert oxygen to carbon dioxide which is absorobed into ethanolamine, causing a net reduction in gas pressure with in the sample flask. The pressure reduction triggers an electrolytic process, generating oxygen and restoring the pressure in the sample flask. The magnitude and duration of the current is proportional to the amount of oxygen supplied to the microorganisms. The data on the oxygen is recorded on computer.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Key result
Parameter:
% degradation (O2 consumption)
Value:
88
Sampling time:
28 d
Parameter:
% degradation (O2 consumption)
Value:
76
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
42
Sampling time:
7 d
Details on results:
10 day window validation criterion passed. The substance is considered readily biodegradable.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The substance is considered readily biodegradable.