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Acute toxicity studies evaluating the toxicity of the substance to aquatic organisms are available for three different trophic levels. All studies were performed according to international guidelines and GLP. Freshwater algae (Pseudokirchneriella subcapitata) turned out to be the most sensitive species with an ErC50 (72 h) of 1.6 mg/L/ErC10 (72 h) of 0.86 mg/L followed by aquatic invertebrates (Daphnia magna; EC50 (48 h): 20 mg/L) and freshwater fish (Danio rerio; LC50 (96 h) > 6.2 mg/L). The toxicity to the microbial community was investigated using the toxicity control from a standard biodegradation study. A degradation of 63% after 14 d did not indicate any toxic effects of the test item to the inoculum at a concentration of 1.5 mg/L. Thus, a NOEC (14 d) of ≥ 1.5 mg/L was derived for the activated sludge microorganisms.