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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride
EC Number:
255-578-9
EC Name:
(3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride
Cas Number:
41892-01-7
Molecular formula:
C17H37ClNO.Cl
IUPAC Name:
(3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride
Specific details on test material used for the study:
40% solution of (3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride

Test solutions

Details on test solutions:
Solutions containing 1.0, 1.8, 3.2, 5.6 and 10 mg/l of the test substance were used as test solutions

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Length: 1.7 +/- 0.2 cm long, Weight: 0.4 +/- 0.01 g

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Hardness:
approx 210 mg/l CaCO3
Test temperature:
24°C +/-1°C
pH:
8.0 - 8.3
Dissolved oxygen:
> 7.5 mg/l

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
7.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
5.6 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Reported statistics and error estimates:
The LC50 results were calculated by means of a parametic model developed by Kooijman (1981)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Numbers of surviving fish at several concentrations of Quab 342 after several exposure times

    Time (h)

                conc. of test substance (mg/l)
 0  1.0  1.8  3.2  5.6  10
 0  10  10  10  10  10  1
 24  10  10

 10

  10  9  0
 48  10  10  10  10  9  0
 72  10  10  10  10  9  0
 96  10(1)  10(2)  10(2)  10(2)  9(2, 3)  0

1) condtion of all fish, visually assessed as good (Normal)

2) conditon of all fish assessed, eual to that of control fish

3) one fish was dead at 5.6 mg/l, but because 10 mortality (in the blanks) is allowed according to OECD 203, this concentration is recorded as the 96h NOEC , with regard to mortality as crtiterion.

Applicant's summary and conclusion

Conclusions:

Results on the 40% test solution give an LC50 (96hr) value of 7.1 mg/l and an NOEC (96hr) value of 5.6 mg/l which equates to 2.84 mg/l of the active ingedient of LC50 (48hr) 2.84 and NOEC (96hr) 2.24 mg/l.