Dodecenylsuccinic acid, compound with 2,2',2''-nitrilotriethanol (1:1)

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because direct and indirect exposure of the soil compartment is unlikely

Justification for type of information:

The substance is used in industrial settings.
The substance rapidly biodegrades and is highly water soluble. Adsorption to sludge and transfer to agricultural soils is not anticipated.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Substance is manufactured in water and considered fully soluble at 40% solution.
On heating to reduce water content, the substance starts to decompose.

Qualifier:

no guideline followed

Principles of method if other than guideline:

The substance is manuafactured as ca 40% in water. An attempt was made to reduce the water level (ie increase concentration > 40%), but on heating, the substance appears to decompose.
The 40% solution is fully miscible in water, with a slight haze (impurities?)

GLP compliance:

no

Type of method:

other: Attempt to concentrate an existing solution

Key result

Water solubility:

> 400 g/L

Conc. based on:

act. ingr.

Temp.:

20 °C

pH:

ca. 6.2

Sample Description Percentage Non-Volatiles

Sample as received 40.33%

Sample after drying 93.92

The results show that the product contains ~60% water, and that ~95% has been removed after drying.

Conclusions:

Considered very soluble in water, but slight haze indicated present of less soluble fractions / impurities

Cross-reference 2

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: screening test, other

Remarks:

Seawater : Closed bottle

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 day

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Guideline study performed to GLP.
This study is known to be more stringent on assessment of biodegradation than screening studies using sewage treatment plant innoculum and a pass in this study is a good indicator of biodegradation potential.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 306 (Biodegradability in Seawater)

Version / remarks:

Closed bottle using natual seawater

Principles of method if other than guideline:

28 day closed-bottle test, using natural seawater.
The seawater was enhanced with trace elements and a low level of phosphates and checks made that microbial counts were within specification.
Nitrification inhibitors were added to prevent distortion of results from nitrate metabolism.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch SRK 184/14
Purity 43%
clear yellow liquid

Oxygen conditions:

aerobic

Inoculum or test system:

natural water: marine

Details on inoculum:

Natural sea water, pH 7.9. Test performed at ca 20 C
The seawater was enhanced with trace elements and a low level of phosphates and checks made that microbial counts were within specification.
Nitrification inhibitors were added to prevent distortion of results from nitrate metabolism.

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

formulation

Initial conc.:

ca. 0.9 mg/L

Based on:

act. ingr.

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

acetic acid, sodium salt

Parameter:

% degradation (DOC removal)

Value:

39

Sampling time:

6 d

Parameter:

% degradation (DOC removal)

Value:

55

Sampling time:

14 d

Parameter:

% degradation (DOC removal)

Value:

55

Sampling time:

20 d

Key result

Parameter:

% degradation (DOC removal)

Value:

61

Sampling time:

28 d

Details on results:

60% acheived over 28 days as percentage of ThOD

Results with reference substance:

Valid, with ca 100% degradation recorded

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

The interprestion as 'readily biodegradable, but failing 10-day window' typically applies to sewage-based screening methods that are typically less difficult to pass. The sewater protocol has a very low microbial count to mimic the impact of spills in marine use.

Therefore a 'pass' in the marine assay is seen as a good indicator of rapid biodegradation in water treatment plants.

Executive summary:

Considered rapidly biodegradable for purposes of classification and is probably passing the 10 -day window if tested by sewage-system assays.