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EC number: 600-065-0 | CAS number: 1003218-40-3
The objective of this study was to determine the effect of the test item on the immobilization of Daphnia magna.
Juvenile daphnids were exposed to a nominal test item concentration of 100 mg/L (limit test) in an open static system. 20 daphnids, divided into four replicates, each with five animals were used in the test item group and the control group. The daphnids were observed for immobilization after 24 and 48 hours of exposure.
The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility ( 7.167 × 10-6 mg/L), the compound cannot be detected with standard analytical methods and the development of an analytical method with a sufficiently low detection and quantification limit is complex.
Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
No effect on the mobility of the daphnia was observed at a nominal concentration of 100 mg/L.
For the test item the following EC50 values were determined:
EC50 (24h) > 7.167 × 10-6 mg/L (nominal >100 mg/L)
EC50 (48h) > 7.167 × 10-6 mg/L (nominal >100 mg/L)
Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of the test item revealed no toxicity to daphnids.The 48h EC50 was > 7.167 × 10-6 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
This study was performed according to GLP and the methods applied are fully compliant with OECD TG 202. Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of the test item revealed no toxicity to daphnids.The 48h EC50 was > 7.167 × 10-6 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
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