Tetralithium 5,5'-[vinylenebis[(3-sulphonato-4,1-phenylene)azo]]bis[3-methylsalicylate]

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Principles of method if other than guideline:

To determine the water solubility of the test item, two different methods can be used according to OECD Guideline 105, EC Guideline A.6 and OPPTS Guideline 830.7840:
− The column elution method for solubility < 10-2 g/L.
− The shake flask method for solubility ≥ 10-2 g/L.
In a preliminary test, the water solubility of the test item was found to be > 10-2 g/L (approximately 345 g/L). Therefore the flask method was chosen for the determination of the water solubility.
For the main test, a quantity of the test item necessary for saturation is dissolved in demineralized water. The vessels are stoppered and stirred on a magnetic stirrer for 24 h, 48 h and 72 h at 30 °C in a temperature controlled water bath. Following this procedure, the temperature is reduced to 20 °C and is kept in the temperature controlled water bath for another 24 h. After phase separation by filtration with a disposable syringe filter and/or centrifugation, the concentration of the test item in the aqueous test solutions is determined by HPLC-analysis with UV-detection.

GLP compliance:

yes

Type of method:

flask method

Specific details on test material used for the study:

Sample number: 2835
Date of receipt: 2016-06-20
Expiry date: 2018-06-17

Water solubility:

240 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

69 other: g / 100 mL

Incubation duration:

24 h

Temp.:

20 °C

pH:

7.5

Remarks on result:

other: Mean value

Water solubility:

254 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

69 other: g / 100 mL

Incubation duration:

24 h

Temp.:

20 °C

pH:

7.5

Water solubility:

234 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

69.1 other: g / 100 mL

Incubation duration:

24 h

Temp.:

20 °C

pH:

7.5

Water solubility:

233 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

68.1 other: g / 100 mL

Incubation duration:

24 h

Temp.:

20 °C

pH:

7.5

Details on results:

The relative standard deviation between the single determinations is < 15 %. The validity criteria have therefore been met.

Conclusions:

The water solubility of Bayscript Gelbkomponente in demineralized water at 20 °C is determined to be 240 g/L according to OECD guideline 105.

Executive summary:

The water solubility of the test item Bayscript Gelbkomponente in demineralized water was determined with the shake flask method at 20 °C according to OECD 105 and OPPTS 830.7840.

The concentration of the test item in the aqueous test solutions was determined by HPLC analysis with UV detection.

Results:

Water solubility test at 20 °C	Flask A [g/L]	Flask B [g/L]	Flask C [g/L]	Mean value
Concentration of Bayscript Gelbkomponente	254	234	233	240 g/L
pH-value	7.5	7.5	7.5	-

The relative standard deviation between the single determinations is < 15 %.

The validity criteria have therefore been met.

Water solubility of Bayscript Gelbkomponente at 20 °C: 240 g/L

Description of key information

The water solubility of the test item Bayscript Gelbkomponente in demineralized water was determined with the shake flask method at 20 °C according to OECD 105 and OPPTS 830.7840.

The concentration of the test item in the aqueous test solutions was determined by HPLC analysis with UV detection.

Results:

Water solubility test at 20 °C	Flask A [g/L]	Flask B [g/L]	Flask C [g/L]	Mean value
Concentration of Bayscript Gelbkomponente	254	234	233	240 g/L
pH-value	7.5	7.5	7.5	-

The relative standard deviation between the single determinations is < 15 %.

The validity criteria have therefore been met.

Water solubility of Bayscript Gelbkomponente at 20 °C: 240 g/L

Key value for chemical safety assessment

Water solubility:

240 g/L

at the temperature of:

20 °C