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EC number: 608-727-0 | CAS number: 323178-01-4
The objective of this study was to evaluate the influence of the test item on the growth and growth rate of the green algae species Desmodesmus subspicatus.
The study design included one control group and one test item group with six replicates, each containing 100 mL reconstituted water or test medium and 10000 cells/mL at the start of the experimental phase.
The study was performed as a limit test in an open static test system. The algae were exposed to a filtrate of nominal 100 mg/L.
The growth of the algae was calculated after 24, 48, and 72 hours exposure in the test item group and control group.
The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (0.0033 mg/L), the compound cannot be detected with standard analytical methods and the development of an analytical method with a sufficiently low detection and quantification limit is complex.
Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
An aqueous preparation of a nominal concentration of 100 mg/L of test item revealed no toxic effect in Desmodesmus subspicatus.
The following EC values (Growth rate and Yield) were determined:
95% confidence interval
>0.0033 mg/L (nominal >100 mg/L)
n.d. = could not be determined
Under the conditions of the present study, an aqueous solution of 100 mg/L of the test item in an open static system revealed no aquatic toxicity. The EC50 (0-72h) for Growth rate and Yield were >0.0033 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
This study was performed according to GLP and the methods applied are fully compliant with OECD TG 201. Under the conditions of the present study, an aqueous solution of 100 mg/L of the test item in an open static system revealed no aquatic toxicity.The EC50(0-72h) for Growth rate and Yield were >0.0033 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
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