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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2005-07-29 to 2005-08-26
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Batch: L540638
Purity: 99.8% (GC)
Vehicle: SPAN 60
Analytical monitoring:
no
Vehicle:
yes
Remarks:
SPAN 60
Details on test solutions:
According to guideline
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): SPAN 60
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)):

- Nominal values

Substance Concentration Solubilizer
pos. Control 0.05 mg/L -
pos. Control 0.5 mg/L -
Test item: 101 mg/L 500 mg/L

Vehilce
Control: - 500 mg/L

Negative
Control - -

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Weight: 0.25 g
Length: 3.2 cm
Lot: 50729
Date: 2005-07-29
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
no
Test temperature:
24.4 +/- 0.5 °C
Nominal and measured concentrations:
101 mg/L
Details on test conditions:
10 fish per 4 L
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 101 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: with vehicle

- Mortality of control: 0 % after 96 hours

Mortality in %

 Sample              Mortality / [%]
 Test Material  Concentration / [mg/L]  24 h  48 h  72 h 96 h
 Positive Control  0.5  20  70  90  100
 Positive Control  0.05  0  0  0  0
 Test item (+vehicle)  101

 0 

 0

 0

 0

SPAN 60

 500

 0

 0

 0

 0

 Blank

 0

 0

 0

 0

 0

All concentrations tested were at or above the solubility limit of the pure test substance. To achive the exposure concentrations, solubiliser was used.

Validity criteria fulfilled:
yes
Conclusions:
The LC50 of the test material exceeds 101 mg/L and is clearly above the limit of water solubility.
Executive summary:

The information for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 203. The LC50 of the test material exceeds 101 mg/L and is clearly above the limit of water solubility.

Description of key information

GLP OECD 203 Assay: NOEC=101 mg/L (measured initial) with solubilizer

Key value for chemical safety assessment

LC50 for freshwater fish:
101 mg/L

Additional information

The LC50 of the test material exceeds 101 mg/L and is clearly above the limit of water solubility.