Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-589-6 | CAS number: 64-67-5
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxic effects of organic solvents on the growth of Chlorella vulgaris and Selenastrum capricornutum.
- Author:
- El Jay, A.
- Year:
- 1 996
- Bibliographic source:
- Bull. Environ. Contam. Toxicol. 57:191-198.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Ethanol
- EC Number:
- 200-578-6
- EC Name:
- Ethanol
- Cas Number:
- 64-17-5
- Molecular formula:
- C2H6O
- IUPAC Name:
- ethanol
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: not described
Test organisms
- Test organisms (species):
- Chlorella vulgaris
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: Chlorella vulgaris
- Source (laboratory, culture collection): Isolated from Lake Geneva in 1980
- Age of inoculum (at test initiation): no data
- Method of cultivation: Axenic using Algal assay Procedure (1971) medium with 15 mg/l NaHCO3 and 12 mg/l K2HPO4 in 500 ml flasks containing 250 ml algal suspension.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 5 d
Test conditions
- Test temperature:
- 21 +/- 1 deg C
- pH:
- Water chemistry (pH) on test: Not described.
- Nominal and measured concentrations:
- Test design: Ethanol was tested three times at each concentration (0, 0.05, 0.1, 0.3, 0.5 and 1% v/v).
Method of calculating mean measured concentrations: Only nominal concentrations were used.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 275 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC50 of the test substance on the growth rate of Chlorella vulgaris at 72 hours was 275 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
