Orthosulfamuron

Toxicity to terrestrial arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

other: SETAC-Guidance document on regulatory testing procedures for pesticides with non-target arthropods

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: ESCORT 2-Guidance on regulatory testing and risk assessment procedures for plant protection products with non-target arthropods

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Test material:
IR5878 50 WG + ADJ1012
Batch number IR5878 50 WG: G 038/02 (49.96 ± 0.17 % w/w)

Test organisms (species):

Aphidius rhopalosiphi

Animal group:

Hymenoptera (leaf-dwelling parasitic wasp)

Details on test organisms:

Test animals:
Taxonomic group: parasitoids (Hymenoptera, Braconidae)
Species: male and female Aphidius rhopalosiphi (De Stephani-Perez)
Age: < 48 hours (at test start)
Housing: 10 individuals per unit (7 female and 3 males during exposure period); 1 female per unit (during post-exposure period)
Food: solution of fructose (25%); acclimatisation: on a cotton wool pad, ad libitum; exposure period: in small test tubes (diameter of approximately 1 cm) which were connected with the exposure units at the beginning of the experiment, ad libitum.

Study type:

laboratory study

Total exposure duration:

48 h

Test temperature:

Temperature: 19 ÷ 21°C (acclimatisation); 19 ÷ 21°C (exposure); 18 ÷ 22°C (post-exposure)

Humidity:

Relative humidity: 75 ÷ 85% (acclimatisation); 75 ÷ 85% (exposure); 60 ÷ 65% (post-exposure; within the test units)

Photoperiod and lighting:

Light: 16 hours light and 8 hours dark; Light intensity: 1070 lux (acclimatisation); 930 ÷ 1030 lux (exposure); 990 ÷ 1380 lux (post-exposure: parasitation period); 9680 ÷ 16680 lux (post-exposure: post-parasitation period)

Nominal and measured concentrations:

Treatment group Mortalitya (%) Corrected mortality (%)
Control 0.0 ± 0.0 -
5.56 g IR5878 50 WG/ha + 37.0 mL ADJ1012/ha 0.0 ± 0.0 0.0
16.7 g IR5878 50 WG/ha + 111 mL ADJ1012/ha 2.5 ± 5.0 2.5
50.0 g IR5878 50 WG/ha + 333 mL ADJ1012/ha 0.0 ± 0.0 0.0
150 g IR5878 50 WG/ha + 1000 mL ADJ1012/ha 0.0 ± 0.0 0.0
450 g IR5878 50 WG/ha + 3000 mL ADJ1012/ha 0.0 ± 0.0 0.0
Toxic standard 100.0 ± 0.0 100.0*
a after 48 hours exposure to spray residues on glass plates; percentage values represent means and standard deviation from 4 replicates each with 7 female and 3 male parasitoids
* p<0.05 (Fisher’s Exact Test)

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 450 g/ha

Conc. based on:

act. ingr.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

LR50

Effect conc.:

> 150 - < 450 g/ha

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

reproduction

Table 8.8-1:    Laboratory test onAphidius rhopalisiphi: mortalities

Treatment group	Mortality^a                             (%)	Corrected mortality             (%)
Control	0.0 ± 0.0	-
5.56gIR5878 50 WG/ha + 37.0 mL ADJ1012/ha	0.0 ± 0.0	0.0
16.7 gIR5878 50 WG/ha + 111 mL ADJ1012/ha	2.5 ± 5.0	2.5
50.0 gIR5878 50 WG/ha + 333 mL ADJ1012/ha	0.0 ± 0.0	0.0
150 gIR5878 50 WG/ha + 1000 mL ADJ1012/ha	0.0 ± 0.0	0.0
450 gIR5878 50 WG/ha + 3000 mL ADJ1012/ha	0.0 ± 0.0	0.0
Toxic standard	100.0 ± 0.0	100.0*
^aafter 48 hours exposure to spray residues on glass plates; percentage values represent means and standard deviation from 4 replicates each with 7 female and 3 male parasitoids * p<0.05 (Fisher’s Exact Test)

 

Treatment group	Parasitation rate^a        (mummies per female)	Reduction of parasitation efficiency^b(%)
Control	34.9 ± 15.1	-
5.56gIR5878 50 WG/ha + 37.0 mL ADJ1012/ha	23.9 ± 12.1	31.5
16.7 gIR5878 50 WG/ha + 111 mL ADJ1012/ha	24.7 ± 11.6	29.2
50.0 gIR5878 50 WG/ha + 333 mL ADJ1012/ha	27.7 ± 17.8	20.6
150 gIR5878 50 WG/ha + 1000 mL ADJ1012/ha	39.3 ± 14.1	- 12.6
450 gIR5878 50 WG/ha + 3000 mL ADJ1012/ha	15.7 ± 11.0	55.0*
^apreviously exposed to spray residues on glass plates; percentage values represent means and standard deviation from 15 replicates each with 1 female ^bnegative value mean increased parasitation efficiency compared to the control * p<0.05 (Fisher’s Exact Test)

Validity criteria fulfilled:

yes

Conclusions:

The LR50 could not be determined due to the low toxicity of test item and is considered higher than 450 g IR5878 50 WG/ha + 3000 mL ADJ1012/ha, the highest dose tested.
The reproductive capacity of the parasitoid Aphidius rhopalosiphi was not statistically significantly reduced up to 150 g IR5878 50 WG/ha + 1000 mL ADJ1012/ha, but was statistically reduced at 450 g IR5878 50 WG/ha + 3000 mL ADJ1012/ha compared to the control

Executive summary:

A study was performed to produce a concentration-response curve for mortality effects seen over 48 hours of exposure to the mixture of IR5878 50 WG + ADJ1012 on the parasitoidAphidius rhopalosiphi. The LR₅₀could not be determined due to the low toxicity of test item and is considered higher thanIR5878 50 WG/ha + 3000 mL ADJ1012/ha, the highest dose tested. The reproductive capacity of the parasitoidAphidius rhopalosiphiwas not statistically significantly reduced up toIR5878 50 WG/ha + 1000 mL ADJ1012/ha, but was statistically reduced atIR5878 50 WG/ha + 3000 mL ADJ1012/ha compared to the control.

Description of key information

A study was performed to produce a concentration-response curve for mortality effects seen over 48 hours of exposure to the mixture of IR5878 50 WG + ADJ1012 on the parasitoid Aphidius rhopalosiphi. The LR₅₀could not be determined due to the low toxicity of test item and is considered higher than IR5878 50 WG/ha + 3000 mL ADJ1012/ha, the highest dose tested. The reproductive capacity of the parasitoid Aphidius rhopalosiphiwas not statistically significantly reduced up toIR5878 50 WG/ha + 1000 mL ADJ1012/ha, but was statistically reduced atIR5878 50 WG/ha + 3000 mL ADJ1012/ha compared to the control.