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EC number: 216-577-9 | CAS number: 1618-26-4
The ready biodegradability of the test item was determined with a non adapted activated sludge over a test period of 28 days in the Closed bottle test design according to OECD 301 D, Regulation (EC) No. 440/2008 Method C.4 -E and EPA OPPTS 835.3110. The test item was tested at a concentration of 2.5 mg/L in duplicates.The oxygen concentration was measured with an O2 electrode. The reference item sodium benzoate was sufficiently degraded to a mean of 82.9 % after 14 days, and to a mean of 87.9% after 28 days of incubation, based on its ThODNH4. The biodegradation of the test item reached a mean of 15.9% after 28 days based on its ThODNH4. In conclusion, the test item is not readily biodegradable as the pass level of 60% degradation was not reached in a 10-d-window within the 28 days period of the study.
The test item is considered to be not ready biodegradable, since the pass level for ready biodegradability (removal of 60% ThODNH4 in a 10-day window) was not fulfilled (reference 5.2.1-1).
The ready biodegradation test was conducted according to the test method OECD 301 D (Closed Bottle Test, reference 5.2.1-1). The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant in completely full and closed bottles in the dark at controlled temperature (22 ± 2 °C) for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. All validity criteria of the study were met. The percentage biodegradation of the test item reached a mean of 15.9% after 28 days based on its ThODNH4. The pass level for ready biodegradability is the removal of 60% ThODNH4 in a 10-day window and therefore the test item is considered to be not ready biodegradable.
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