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EC number: 216-577-9 | CAS number: 1618-26-4
The water solubility of the test item estimated to be was 3.6 g/L in a preliminary test and therefore the shake-flask method was used in the main test.
The calibration solutions were prepared in 0.2 – 100 μg/mL range. The calibration samples were analysed by HPLC-UV method with three replicate injections from each. Measured concentrations of the test item are summarised in Table 1. The repeatability of the determination was < 15% and thus within the range required by the guidelines.
Table 1: Results of the determination of water solubility of the test item
A study was conducted according to OECD TG 105, Regulation (EC) No. 440/2008 method A.6 and OPPTS 830.7840 in order to determine the water solubility of the test item. Since in the preliminary test the water solubility was 3.6 g/L the shake flask method was used for the main test. The calibration solutions were prepared in 0.2 – 100 μg/mL range. The calibration samples were analysed by HPLC-UV method with three replicate injections from each. The water solubility of test item was determined to be 3.12 ± 0.05 g/L (95% confidence interval) at 20 °C ± 0.5 °C and 72 h sampling time (reference 4.8-1).
The water solubility of test item was determined to be 3.12 ± 0.05 g/L (95% confidence interval) at 20 °C ± 0.5 °C using the shake-flask method for the performance of the main test.
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