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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Feb 24 - June 19 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was ground, and added to a weighed beaker. The test substance was then added to 13 L of dilution water. The solution was then mixed for 48 hrs, and then allowed to settle for 5 hrs.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Precipitate was present in the dilution solution. Great care was taken when decanting the dilution water into the test chamber to avoid transferring precipitate. No evidence of precipitate was seen in the test chambers.
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: fathead minnow
- Source: Chesapeake Cultures, Inc., Hayes, Virginia, USA
- Age at study initiation (mean and range, SD): juveniles
- Length at study initiation (length definition, mean, range and SD): 2.4-2.8 cm, average 2.6 cm (control fish at end of study)
- Weight at study initiation (mean and range, SD): 0.09-0.15 g, average 0.12 g (control fish at end of study)

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: brine shrimp nauplii
- Feeding frequency during acclimation: daily, except for two days prior to the test

FEEDING DURING TEST
- Food type: brine shrimp nauplii
- Frequency: Daily, except for two days prior to the test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
148 mg/L as CaCO3
Test temperature:
22 +/- 1 °C
pH:
7.9-8.3
Dissolved oxygen:
>= 6.2 mg/L
Conductivity:
355 µS/cm
Nominal and measured concentrations:
Nominal: control, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 25 L
- Material, size, headspace, fill volume: stainless steel, 25 L size filled to 12 L
- No. of organisms per vessel: 7
- Biomass loading rate: 0.063 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 40 m deep well on laboratory site
- Total organic carbon: 2.0-5.2 mg C/L
- Metals: No metals were present above the detection limits.
- Pesticides: No pesticides were present above the detection limits.
- Chlorine: 4.3 mg/L
- Alkalinity: 180 mg/L as CaCO3
- Ca/mg ratio: 2.6

OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs darkness with 30 min transition period
- Light intensity: 350 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, abnormal behaviour with observation intervals at 18, 24, 48, 72, and 96 hrs after start of exposure
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: None
- Mortality of control: None
- Abnormal responses: None

Results

 Observation  Mortality - Control Group  Mortality - 100 mg/L Group
 18 hrs  0  0
 24 hrs  0  0
 48 hrs  0  0
 72 hrs  0  0
 96 hrs  0  0
Validity criteria fulfilled:
yes
Conclusions:
The 96-hr LL50 in fathead minnow is > 100 mg/L (WAF).
Executive summary:

The acute toxicity of sucrose esters to fish was determined in an OECD Guideline 203 study. Groups of seven fish were exposed to 0 or 100 mg/L of sucrose esters for 96 hrs. Due to the complex nature of the test substance, results were reported as water accomodated fraction (WAF). No fish in the test or control groups died or showed abnormal behaviour. The 96-hr LL50 is therefore >100 mg/L (WAF).

Description of key information

LL50 (96 h) > 100 mg/L (nominal, OECD 203)

Key value for chemical safety assessment

Additional information

One experimental study is available investigating the toxicity of the test item to the freshwater fish Pimephales promelas. The study was performed according to OECD 203 (GLP) under static conditions. Due to the low water solubility the test solutions were prepared as Water Accommodated Fractions (WAF) and the loading rate of 100 mg/L (nominal) was tested. The WAF was prepared by adding an appropriate amount of test item to test medium followed by mixing for 48 h and a settling time of 5 h. Since undissolved particles were present in the solution it was carefully decanted to the exposure vessels in order to avoid undissolved material in the test vessels. No evidence of precipitate was seen in the test chambers. After exposure of 96 h no mortality was recorded resulting in a LL50 (96 h) of > 100 mg/L based on the nominal loading rate.