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EC number: 600-809-4 | CAS number: 1072-53-3
Inhibition of RespirationRate
The following results were derived:
ESA (1,3,2-Dioxathiolane, 2,2-dioxide)
ECx(3 Hours) (mg/L)
95% Confidence Limits (mg/L)
7.0 - 11
pH Values of the Test Item Stock Solutions prior to theAdditionof Inoculum in the Range-FindingTest
Nominal Concentration (mg/L)
Prior to Adjustment
*pH meter did not give a reading as the pH of the stock solution was out of range for the pH meter. The pH reading on the meter dropped before showing that the pH was out of range indicating that the stock solution was acidic. The manufacturer’s manual for the pH meter indicates that the pH meter will not take readings below pH 2. Therefore it is reasonable to assume that the pH of the 2500 mg/L stock solution was below pH 2
Dissolved Oxygen Concentrations of the TestPreparationsafter 30 Minutes Contact Time in the Range-FindingTest
Dissolved Oxygen Concentration (mg O2/L)
Dissolved Oxygen Saturation Level of 8.9 mg O2/L
R = Replicate
Oxygen Consumption Rates and Percentage InhibitionValuesafter 3 Hours Contact Time in the Range-FindingTest
Initial O2Reading (mgO2/L)
Measurement Period (minutes)
Final O2Reading (mgO2/L)
R = Replicate
- = Notapplicable
[ ] = Increase in respiration rate as compared to controls
A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)".
Activated sewage sludge was exposed to an aqueous solution of the test item at concentrations of 10, 100 and 1000 mg/L (three replicates of the 1000 mg/L test concentration) for a period of 3 hours at measured temperatures of approximately 20 °C with the addition of a synthetic sewage as a respiratory substrate.
The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-Dichlorophenol.
The effect of the test item on the respiration of activated sewage sludge gave a 3-Hour EC50 value of greater than 1000 mg/L.
It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/L.
The reference item gave a 3-Hour EC50 value of 8.9 mg/L, 95% confidence limits 7.0 to 11 mg/L.
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