Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal waste water treatment plant Mannheim, Germany
- Preparation of inoculum for exposure: Inoculum was pre-aerated two days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
10 mg/L
Based on:
TOC
Initial conc.:
mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Test temperature: 22 ± 2 °C

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for two days before test start
- Measuring equipment: carbon dioxide trap
- Test performed in open system: no

SAMPLING
- Sampling frequency: every other day

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Inhibition control
Reference substance
Reference substance:
aniline
Remarks:
20 mg/L TOC

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
0
Sampling time:
28 d

Applicant's summary and conclusion