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EC number: 450-320-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 14 September, 2017 to 17 October, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 450-320-3
- EC Name:
- -
- Molecular formula:
- C15H28O
- IUPAC Name:
- (3S,6E)- 3,7,11-trimethyldodeca-6,10-dien-1-ol
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material:6K0001
- Purity test date:97.5%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:The test sample was stored in a dark storage place at room
temperature.
- Stability under test conditions: IR spectrum of the test item after the completion of the experiment
was the same as that before the start of the experiment, and it indicated that the test item was stable
under the storage conditions.
- Solubility and stability of the test substance in the solvent/vehicle: Dimethylsulfoxide;>50 mg/mL,
Acetone;>50 mg/mL
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Stock solution content of 100, 31.6, 10.0, 3.16 and 1.00% (all test levels)
- Sampling method: Another solution sampled separately from the preparation container (at the start
of exposure)
The mixed solution taken out with equal volume of the test solution from the test vessels in each test
level (at the end of exposure)
- Sample storage conditions before analysis: immidiately pretreated
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:test sample and medium were mixed to prepare the nominal concentration of 100 mg/L and
stirred for 48 hours. The test solution was prepared by diluting the stock solution with medium.
- Controls:medium without the test item, which was treated in the same manner as the stock solution
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Source; American Type Culture Collection
Strain number; ATCC 22662
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- OECD medium
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23.0-23.2 derees(Celsius)
- pH:
- 7.8-9.4
- Nominal and measured concentrations:
- Stock solution content of 100, 31.6, 10.0, 3.16 and 1.00% (2.76, 0.813, 0.271, 0.0774 and 0.0247 mg/
L as measured concentrations) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterilized 500 mL Erlenmeyer flask (closed vessel)
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Volume of the test solution; 100 mL (headspace more than
400 mL)
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): none
- Renewal rate of test solution (frequency/flow rate):none
- Initial cells density: 5000 cells/mL
- Control end cells density: 350000 cells/mL(mean)
- No. of organisms per vessel: 5000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):6
- No. of vessels per vehicle control (replicates):0
GROWTH MEDIUM
- Standard medium used: yes(OECD TG medium)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified water
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH:none
- Photoperiod:continuous illumination
- Light intensity and quality:nominal 90μmol∙m-2∙s-1 (87 to 89 μmol∙m-2∙s-1)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : every 24 hours after
the start of exposure
- Determination of cell concentrations: [ electronic particle counter; ]
- Chlorophyll measurement:none
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.16
- Range finding study
- Test concentrations: stock solution content of 100, 32.0, 10.0, 3.20, 1.00, 0.320%
- Results used to determine the conditions for the definitive study:test range - Reference substance (positive control):
- not specified
- Remarks:
- There are periodically reference substance data.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.43 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.077 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Unusual cell shape:Some bloat cells were observed in 2.76 mg/L level.
- Colour differences: none
- Flocculation: none
- Adherence to test vessels: none
- Aggregation of algal cells: none
- Any stimulation of growth found in any treatment: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal
values:none
- Effect concentrations exceeding solubility of substance in test medium:none - Results with reference substance (positive control):
- - EC50:1.1 mg/L(Potassium dichromate)
- Reported statistics and error estimates:
- Regarding the growth rate, Bartlett’s test was done to determine the homogeneity of variance for the
data. Then one-way analysis of variance and Dunnett’s multiple comparison test was used to determ
ine the significant difference between the control and the exposure levels. Higher concentration level
than ErC50 on respective parameter were excluded for each analysis.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- ErC50 was 1.43 mg/L and NOEC was 0.0774 mg/L.
- Executive summary:
This study was conducted in order to estimate the effect on the test organisms under the solubility of
test item in medium.The test item has a smell. In preliminary study, it was found that the test item of test
solution in closed vessel during the exposure was more stable than the test vessel with gas-permeable
Silicosen®. Therefore, definitive study was conducted using the closed vessel.As a result, ErC50 was
1.43 mg/L and NOEC was 0.0774 mg/L. The test item concentration in test solution was reduced slightly
during the exposure, and that at the end of exposure was 63.6-87.8% of the concentration at the start. So,
the results of study were estimated by geometric mean of the measured concentrations of test item in
test solution during the exposure.
The environmental conditions were within the suitable range. Therefore, it is concluded that this study
complied with the applied test guideline.
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