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EC number: 612-028-6 | CAS number: 607724-47-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- • OECD Guidelines for the Testing of Chemicals, Part 301 B, adopted 17. Jul. 1992
CO2-Evolution-Test (Modified STURM Test)“
• Council Regulation (EC) No. 440/2008, Method C.4-C, adopted 30. May 2008 “CO2-Evolution-Test” - Deviations:
- no
- Remarks:
- A deviation from the study plan was documented:
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly domestic sewage
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 21. Apr. 2017, batch no: 20170421
The sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated until use.The dry matter was determined as 4040 mg suspended solids/L.
Preparations:
The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its DOC was measured. The stock solution of the test item was prepared and its DOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2 - Duration of test (contact time):
- >= 28 d
- Initial conc.:
- ca. 20 mg/L
- Based on:
- IC (inorganic carbon)
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its DOC was measured. The stock solution of the test item was prepared and its DOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2
1)Apparatus
The test vessels were aerated with purified (by activated charcoal), CO2-scrubbed, mois-tened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5 M NaOH. To control the absence of CO2, the air was then led through a flask containing a solution of Ba(OH)2 before reaching the test vessels.
Magnetic stirrers were used to prevent deposition of inoculum.
The emitted CO2 was trapped in 0.25 M NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels. The initial IC value of the 0.25 M NaOH was separately determined in each flask.
2) Sampling
From each front scrubber flask, 9 samples were taken in order to determine the emitted CO2 (on day 0, 2, 4, 8, 10, 14, 18, 23 and 29). The sample volume was 1 mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO2 (see also chapter 8.3.1).
On day 28, 5 mL HCl 2 M was added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken.
3) CO2 Determination
Analyses of the emitted CO2 were made by IC measurement using the carbon analyser TOC multi N/C 2100S, Analytik Jena. Each sample was measured in duplicate or tripli-cate, respectively (depending on the variation between the measured values). The carbon analyser was calibrated with freshly prepared reference solutions containing potassium hydrogen phthalate (TC), sodium hydrogen carbonate and sodium carbonate (IC) every month. After every start, quality control samples were measured. - Reference substance:
- aniline
- Remarks:
- positive control
- Preliminary study:
- In a non-GLP pre-test, the carbon content of a stock solution of the test item was meas-ured in the membrane filtered solution and in the unfiltered solution. The measured con-centrations were in a similar range. For the test a stock solution containing 1000.4 mg/L was prepared. Its carbon content was determined in the unfiltered solution in order to es-timate the amount to be added to the test flasks.
The DOC was 278.15 mg/L, giving an organic carbon content of 27.8 %. - Test performance:
- Preparations
The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its DOC was measured. The stock solution of the test item was prepared and its DOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2. - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 0
- Sampling time:
- 28 d
- Results with reference substance:
- not readily biodegradable
- Validity criteria fulfilled:
- yes
- Remarks:
- No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item Blendazol Red Blendwell is considered as “not readily biodegrada-ble“.
For the test item Blendazol Red Blendwell no biodegradation was observed after 28 days. The 10-day window could not be determined
The criterion of reaching 60% of degradation after 28 days was not met. Blendazol Red Blendwell is therefore con-sidered as
“notreadilybiodegradable”.
The abiotic degradation reached 0.7 %. - Executive summary:
The aerobic ready biodegradability of Blendazol Red Blendwell in the CO2Evolution Test was determined following OECD 301B resp.
EU C.4-C, the biodegradability was determined after a duration of 28 days
The following data were determined for the test item Blendazol Red Blendwell:
10-day-window: not detected
degradation at the end of 10-day-window 0 %
degradation at the end of the test 0 %
pass level following guideline: 60% at the end of 10-day-window for purerespective 60 % at the end of the test for mixtures
For the test item Blendazol Red Blendwell no biodegradation was observed after 28 days.
Blendazol Red Blendwell is therefore considered a“not readily biodegradable”.
Reference
1.1.1 Degradation Values
In the following table, the percentage biodegradation is presented:
Degradation values in %
Day |
Positive Control 1 |
Positive Control 2 |
Positive Control Mean |
Test 1 |
Test 2 |
Test Mean |
Abiotic Control |
Toxicity Control |
2 |
-0.6 |
1.5 |
0.5 |
0.6 |
-1.2 |
-0.3 |
0.6 |
1.5 |
4 |
17.9 |
21.2 |
19.5 |
0.4 |
0.3 |
0.3 |
1.7 |
7.0 |
8 |
55.6 |
69.1 |
62.3 |
0.3 |
0.5 |
0.4 |
1.6 |
26.7 |
10 |
68.8 |
80.7 |
74.7 |
-0.2 |
1.9 |
0.9 |
1.4 |
30.0 |
14 |
84.4 |
86.7 |
85.5 |
0.3 |
0.9 |
0.6 |
0.8 |
34.0 |
18 |
85.0 |
84.7 |
84.8 |
-2.0 |
-1.3 |
-1.6 |
1.0 |
34.0 |
23 |
86.8 |
85.4 |
86.1 |
-1.9 |
-1.8 |
-1.9 |
0.7 |
34.2 |
29 |
86.4 |
82.9 |
84.6 |
-5.3 |
-5.3 |
-5.3 |
0.7 |
33.1 |
Because the values of day 29 are the sum of the IC values in scrubber flasks A and B, an increase (IC values in flasks B of the test flasks higher than in those of the control) or a decrease (IC values in flasks B of the test flasks lower than in those of the control) of degradation can be observed.
As the measured IC values in the test flasks are very low, measurement uncertainties lead to negative degradation values while in
fact no degradation has taken place
Description of key information
Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation reached 0.7 %. Both replicates of the test item showed very good correspondence.
If degradation in the toxicity flask is below 25% after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 34.0% after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 72.0 mg/L”.
Ready biodegradability is defined in the guidelines as degradation surpassing 60% within 10 days after reaching a level of 10%.
For the test item Blendazol Red Blendwell no biodegradation was observed after 28 days.Blendazol Red Blendwell is therefore considered as “not readily biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
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