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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

72 h ErC10: 19 mg/L
72 h ErC50: 98 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
98 mg/L
EC10 or NOEC for freshwater algae:
19 mg/L

Additional information

In the Klimisch 1 GLP study from Vryenhoef (2016) the toxicity of Butanedioic acid, sulfo-, 4-[2 -[(1 -oxo-10 -undecenyl)amino]ethyl] ester, disodium salt to Pseudokirchneriella subcapitata was determined in a static 72 Hour Algal Growth Inhibition Test. The test item was an aqueos solution/suspension of the test substance. The test concentrations were based on active ingredient. The test was performed according to OECD 201 and EU Method C.3. The nominal concentrations of 1.0, 3.2, 10, 32 and 100 mg ai/L were tested in parallel with a control. Dose verification analysis was performed. Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to be near nominal with the exception of the 3.2 mg ai/L test sample at 72 hours where a measured concentration of 185% of nominal was obtained. This was considered to be due to an error during the analytical work up given that a measured concentration of 99% of nominal was obtained at 0 hours. Given this it was considered appropriate to calculate the results based on nominal test concentrations only.

The cell density was determined at 0, 24, 48 and 72 hours. The cell densities determined using a Coulter® Multisizer Particle Counter. Three determinations were made for each sample. The nominally inoculated cell concentration (5.00 x 10^3 cells/mL) was taken as the starting cell density.

The growth of the control cultures fulfilled the validity criteria from OECD 201 (2006). The 72 -hour ErC10 and ErC50 are 19 and 98 mg a.i./L based on nominal concentration.

The results of this test are considered relevant and reliable for the risk assessment of the registered substance.

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