6,6'-(Ethane-1,2-diylbis(azanediyl))bis(dibenzo[c,e][1,2]oxaphosphinine-6-oxide)“

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2014-10-08 - 2014-10-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

The study was performed by the Swiss Federal Laboratories for Materials Science and Technology (Empa), an it is an institution of the Swiss federation. The study was performed according to OECD 2012 but without GLP which does not affect the reliability of this study.

GLP compliance:

no

Remarks:

The study was performed by the Swiss Federal Laboratories for Materials Science and Technology (Empa), an it is an institution of the Swiss federation.

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Since EMPA FR1 (EDA-DOPO) is poorly soluble, the water accommodated fraction (WAF) approach was used. The loadings were prepared by adding the respective amounts of test item to empty glass vessels, adding the aerated Daphnia medium, moderately stirring it for 24 h, and finally filtering it (MILLIPORE AP15 glass fiber filter). The resulting water soluble fractions (WSF) were used in the test.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia (Straus, 1820), aged less than 24 h, were acclimatized to the test medium for about 1 h before introduction into the formulated test media.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.52 mmol/l

Test temperature:

20±2°C

pH:

6.9 - 7.0

Dissolved oxygen:

6.9 - 7.4 mg/l

Conductivity:

711-794 µS *cm-1

Nominal and measured concentrations:

nominal: Water accomodated fraction loading rates: 10, 100, 1000 mg/l

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

		Number of	Immobile	Immobile	% immobile	% immobile
Loading rate (mg/l)	Replicate	individuals per test	Daphnia(No./vessel)	Daphnia(No./vessel)	Daphnia after 24 h of	Daphnia after 48 h of
		vessel	24h	48h	exposure	exposure
	A	20	0	0	0	0
Control0	B	20	0	0	0	0
	C	20	0	0	0	0
10	A	20	0	0	0	0
	B	20	0	0	0	0
100	A	20	0	0	0	0
	B	20	0	0	0	0
1000	A	20	0	1	0	5
	B	20	2	2	10	10

Validity criteria fulfilled:

yes

Conclusions:

The 24 and 48 hour LC50 of EDA-DOPO was determined in an acute Daphnia immobilization test according to OECD Guideline 202. 24 hour old Daphnids were exposed to the test item as a water accomodated fraction. The 24 and 48-hour LC 50 was determined as > 1,000 mg/l.

Executive summary:

The median effective concentration (EC₅₀) and the no-observed effect concentration (NOEC) of EMPA FR1 (EDA-DOPO) to Daphnia magnawere investigated under static exposure conditions over a period of 48 h. 40 individual Daphnia divided into 2 test vessels were exposed to the test item. Since EMPA FR1 (EDA-DOPO) is poorly soluble, the water accommodated fraction (WAF) approach was applied. No analytical verification of the test item was conducted. The evaluation of the test was based on the loading rates of the test item, assuming the test item to be stable in water over 48 h.The applied loading rates were 10, 100 and 1000 mg/l. After 24 and 48 h of incubation no significant effects (<10% of a total of 40 individuals) were observed at any of the 10, 100 and 1000 mg/l loadings rates and in the blank control.The medium effect concentration (EC₅₀) for EMPA FR1 (EDA-DOPO) after 24 and 48 h of exposure was estimated to be >1000 mg/l loading rate.The no-observed effect concentration (NOEC) after 24 h and 48 h of exposure was shown to be 1000 mg/l loading rate. Commission Regulation (EU) No 286/2011 (i.e. CLP regulation) requires the use of the EC₅₀48 h value for classification purposes. Based on an EC₅₀48 h >100 mg/l,EMPA FR1 (EDA-DOPO) is neither regarded as toxic nor harmful to the daphnid Daphnia magna.

Description of key information

The 24 and 48 hour LC50 of EDA-DOPO was determined in an acute Daphnia immobilization test according to OECD Guideline 202.  24 hour old  Daphnids were exposed to the test item as a water accomodated fraction. The 24 and 48-hour LC 50 was determined as > 1,000 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 000 mg/L